Invitrogen

NUP107 Recombinant Rabbit Monoclonal Antibody (8E6H4)

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NUP107 Antibody (MA5-55218) in WB

Western Blot analysis of various lysates using NUP107 Recombinant Monoclonal Antibody (Product # MA5-55218) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

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产品信息

MA5-55218

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:500

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

8E6H4

抗原

A synthetic peptide corresponding to a sequence within amino acids 700-800 of human Nup107.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.3, with 50% glycerol, 0.05% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Dry ice (international)

产品详细信息

Immunogen Sequence: LASKKHEAAK EVFVKIPQDS IAEIYNQCEE QGMESPLPAE DDNAIREHLC IRAYLEAHET FNEWFKHMNS VPQKPALIPQ PTFTEKVAHE HKEKKYEMDF G

靶标信息

The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. The mammalian nucleoporin (NUP)-107 is part of the hetero-oligomeric complex that also contains NUP160, NUP133, NUP96, and mammalian homolog of yeast sec13p. While the majority of the NUP107-160 nuclear pore sub-complex localizes to the nuclear pore, a small fraction is observed at kinetochores and pro-metaphase spindle poles in mitotic cells in association with proteins such as Mad1, Mad2, Bub3 and Cdc20. Immunodepletion of the NUP107-160 complex resulted in defective spindle assembly indicating that it has multiple functions. NUP107 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP107 may be an important drug target in HIV treatment. Multiple isoforms of NUP107 are known to exist.

仅用于科研。不用于诊断过程。未经明确授权不得转售。