Invitrogen

NUP160 Polyclonal Antibody

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NUP160 Antibody (PA5-88967) in WB

Western blot analysis of NUP160 in extracts of various cell lines. Samples were incubated with NUP160 Polyclonal antibody (Product # PA5-88967) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

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产品信息

PA5-88967

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 40-150 of human NUP160 (NP_0560461)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.52 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2805262

产品详细信息

Immunogen sequence: ALERSFVELS GAERERPRHF REFTVCSIGT ANAVAGAVKY SESAGGFYYV ESGKLFSVTR NRFIHWKTSG DTLELMEESL DINLLNNAIR LKFQNCSVLP GGVYVSETQN R; Positive Samples: A-431, Jurkat, HepG2; Cellular Location: Nucleus, nuclear pore complex

靶标信息

The nuclear pore complex (NPC) is a protein assembly localized at the nuclear rim and mediates macromolecular transport between the nucleus and the cytoplasm. The mammalian nucleoporin (NUP)-160 is part of the hetero-oligomeric complex that also contains NUP107, NUP133, NUP96, and mammalian homolog of yeast sec13p. While the majority of the NUP107-160 nuclear pore sub-complex localizes to the nuclear pore, a small fraction is observed at kinetochores and pro-metaphase spindle poles in mitotic cells in association with proteins such as Mad1, Mad2, Bub3 and Cdc20. Immunodepletion of the NUP107-160 complex resulted in defective spindle assembly indicating that it has multiple functions. NUP160 has recently been identified as an HIV dependency factor (HDF), suggesting that NUP160 may be an important drug target in HIV treatment. Multiple isoforms of NUP160 are known to exist.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。