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NUP214 Antibody (A300-716A-T) in WB
Detection of human and mouse NUP214 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), and 293T (50µg) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NUP214 antibody A300-716A-M (lot A300-716A-M-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
产品信息
A300-716A-T
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Between 1250 and 1300
偶联物
形式
Liquid
浓度
200 µg/mL
纯化类型
Antigen affinity chromatography
保存液
TBS, pH 7.0 to 8.0, with 0.1% BSA
内含物
0.09% sodium azide
保存条件
4° C
运输条件
Wet ice
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
靶标信息
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. This gene is a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is localized to the cytoplasmic face of the nuclear pore complex where it is required for proper cell cycle progression and nucleocytoplasmic transport. The 3' portion of this gene forms a fusion gene with the DEK gene on chromosome 6 in a t(6,9) translocation associated with acute myeloid leukemia and myelodysplastic syndrome. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
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生物信息学
蛋白别名: 214 kDa nucleoporin; CAN protein, putative oncogene; Nuclear pore complex protein Nup214; nucleoporin 214kDa; Nucleoporin Nup214; Protein CAN
基因别名: CAIN; CAN; KIAA0023; NUP214
UniProt ID: (Human) P35658
Entrez Gene ID: (Human) 8021
RNA export from nucleus
mRNA export from nucleus
tRNA export from nucleus
protein import into nucleus
protein export from nucleus
mitotic nuclear envelope disassembly
regulation of glucose transport
viral process
protein sumoylation
viral transcription
gene silencing by RNA
regulation of mRNA stability
regulation of cell cycle
intracellular transport of virus
regulation of cellular response to heat
