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NUP98 Antibody (MA5-38425) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of NUP98 in HeLa cells using NUP98 Monoclonal Antibody (Product # MA5-38425) diluted at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
产品信息
MA5-38425
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
2H4
抗原
A synthesized peptide derived from human NUP98
偶联物
形式
Liquid
浓度
0.6 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol, 150mM NaCl
内含物
0.02% sodium azide
保存条件
-20°C or -80°C if preferred
运输条件
Wet ice
RRID
AB_2898339
产品详细信息
This antibody has been tested in direct-ELISA
靶标信息
Signal-mediated nuclear import and export proceed through the nuclear pore complex (NPC), which is comprised of approximately 50 unique proteins collectively known as nucleoporins. The 98 kDa nucleoporin is generated through a biogenesis pathway that involves synthesis and proteolytic cleavage of a 186 kDa precursor protein. This cleavage results in the 98 kDa nucleoporin as well as a 96 kDa nucleoporin, both of which are localized to the nucleoplasmic side of the NPC. Rat studies show that the 98 kDa nucleoporin functions as one of several docking site nucleoporins of transport substrates. The human gene has been shown to fuse to several genes following chromosome translocations in acute myelogenous leukemia (AML) and T-cell acute lymphocytic leukemia (T-ALL). This gene is one of several genes located in the imprinted gene domain of 11p15. 5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: GLFG-repeat containing nucleoporin; Nuclear pore complex protein Nup98-Nup96; nucleoporin 98kD; nucleoporin 98kDa; Nup98-Nup96; NUP98/PHF23 fusion 2 protein; OTTHUMP00000013954
基因别名: ADAR2; ADIR2; NUP196; NUP96; NUP98
UniProt ID: (Human) P52948
Entrez Gene ID: (Human) 4928
protein import into nucleus, docking
posttranscriptional tethering of RNA polymerase II gene DNA at nuclear periphery
DNA replication
RNA export from nucleus
mRNA export from nucleus
tRNA export from nucleus
nucleocytoplasmic transport
nuclear pore organization
sister chromatid cohesion
mitotic nuclear envelope disassembly
regulation of glucose transport
viral process
protein sumoylation
viral transcription
gene silencing by RNA
telomere tethering at nuclear periphery
nuclear pore complex assembly
intracellular transport of virus
regulation of cellular response to heat
