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Application
FIGURE: 1 / 2
Naked1 Antibody (PA5-113280) in ICC/IF
Immunocytochemichal analysis of Naked1 in HepG2 cells using a Polyclonal antibody (Product # PA5-113280) at a dilution of 1:200. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were also counter-stained with DAPI.
Product Details
PA5-113280
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Protein naked cuticle homolog 1 protein (269-384AA)
Conjugate
Unconjugated
Form
Liquid
Concentration
3.52 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2868014
Target Information
In the mouse, Nkd is a Dishevelled (see DVL1; MIM 601365)-binding protein that functions as a negative regulator of the Wnt (see WNT1; MIM 164820)-beta-catenin-Tcf signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dvl-binding protein; hNkd; hNkd1; naked cuticle 1 homolog; naked cuticle-1; Naked-1; Protein naked cuticle homolog 1
Gene Aliases: Naked1; NKD; NKD1; PP7246
UniProt ID: (Human) Q969G9
Entrez Gene ID: (Human) 85407, (Rat) 364952
negative regulation of Wnt signaling pathway
positive regulation of protein catabolic process
negative regulation of canonical Wnt signaling pathway
positive regulation of non-canonical Wnt signaling pathway via JNK cascade
negative regulation of convergent extension involved in axis elongation
positive regulation of Wnt signaling pathway, planar cell polarity pathway
eye photoreceptor cell differentiation
somatic muscle development
Wnt signaling pathway
regulation of cell motility involved in somitogenic axis elongation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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