Loading ...

Invitrogen

Nesprin 1 Monoclonal Antibody (MANNES1A(7A12))

5个已发表图片

10 篇参考文献

查看其他10个Nesprin 1抗体

说明书

实验方案

技术支持

货号 MA5-18077

CNY 6,592.00

100 µL

查看价格

批量或定制请求

说明书

实验方案

技术支持

抗体检测数据 (2)
已发表图片 (5)

Application

Nesprin 1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

Nesprin 1 Antibody (MA5-18077) in ICC/IF

Immunofluorescent analysis of Nesprin 1 in normal human fibroblast cells (green) using a Nesprin 1 monoclonal antibody (Product # MA5-18077); chromosomes were stained using DAPI (blue).

Product Image Gallery

产品信息

MA5-18077

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:400-1:800

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:200

查看7篇已发表文章 7篇已发表文章

免疫电镜技术 (IM)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human, Mouse, Rabbit, Rat

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

MANNES1A(7A12)

抗原

Recombinant full length Nesprin 1(Human)

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2539460

产品详细信息

This antibody recognizes the C-terminal region of nesprin-1.

This target has a predicted molecular weight of 1011 kDa.

靶标信息

There are two genes encoding members of a new family of type II integral membrane proteins. Both are ubiquitously expressed, and tissue-specific alternative mRNA initiation and splicing generate at least two major isoforms of each protein, with the smaller isoforms being truncated at the N-terminus. These proteins are called nesprin-l and -2 for nuclear _envelope _spectrin repeat, as they are characterized by the presence of multiple, clustered spectrin repeats, bipartite nuclear localization sequences and a conserved C-terminal, single transmembrane domain. Transient transfection of EGFP-fusion expression constructs demonstrated their localization to the nuclear membrane with a novel C-terminal, TM-domain-containing sequence essential for perinuclear localization. Nesprin-l is developmentally regulated in both smooth and skeletal muscle and is relocalized from the nuclear envelope to the nucleus and cytoplasm during C2Cl2 myoblast differentiation. Nesprins may function as 'dystrophins of the nucleus' to maintain nuclear organization and structura integrity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Disulfide bond in SUN2 regulates dynamic remodeling of LINC complexes at the nuclear envelope.

Life science alliance

Sharma R,Hetzer MW

MA5-18077 was used in Immunocytochemistry-immunofluorescence to report a conformation-specific SUN2 antibody as a tool to visualize LINC complex dynamics in situ.

Tue Aug 01 00:00:00 UTC 2023

The LINC complex is required for endothelial cell adhesion and adaptation to shear stress and cyclic stretch.

Molecular biology of the cell

Denis KB,Cabe JI,Danielsson BE,Tieu KV,Mayer CR,Conway DE

Published figure using Nesprin 1 monoclonal antibody (Product # MA5-18077) in Immunocytochemistry

Thu Aug 19 00:00:00 UTC 2021

B Cells Adapt Their Nuclear Morphology to Organize the Immune Synapse and Facilitate Antigen Extraction.

Frontiers in immunology

Ulloa R,Corrales O,Cabrera-Reyes F,Jara-Wilde J,Saez JJ,Rivas C,Lagos J,Härtel S,Quiroga C,Yuseff MI,Diaz-Muñoz J

MA5-18077 was used in Western Blot, Immunocytochemistry to show that upon activation, B cells re-orientate and adapt the size of their nuclear groove facing the immune synapse, where the MTOC sits, and lysosomes accumulate.

Fri Apr 08 00:00:00 UTC 2022

B Cells Adapt Their Nuclear Morphology to Organize the Immune Synapse and Facilitate Antigen Extraction.

Frontiers in immunology

Ulloa R,Corrales O,Cabrera-Reyes F,Jara-Wilde J,Saez JJ,Rivas C,Lagos J,Härtel S,Quiroga C,Yuseff MI,Diaz-Muñoz J

Fri Apr 08 00:00:00 UTC 2022

B Cells Adapt Their Nuclear Morphology to Organize the Immune Synapse and Facilitate Antigen Extraction.

Frontiers in immunology

Ulloa R,Corrales O,Cabrera-Reyes F,Jara-Wilde J,Saez JJ,Rivas C,Lagos J,Härtel S,Quiroga C,Yuseff MI,Diaz-Muñoz J

Fri Apr 08 00:00:00 UTC 2022

Mechanical suppression of breast cancer cell invasion and paracrine signaling to osteoclasts requires nucleo-cytoskeletal connectivity.

Bone research

Yi X,Wright LE,Pagnotti GM,Uzer G,Powell KM,Wallace JM,Sankar U,Rubin CT,Mohammad K,Guise TA,Thompson WR

MA5-18077 was used in Immunocytochemistry-immunoflourescence to examine the mechanisms by which cancer cells sense and respond to low-magnitude mechanical signals introduced from vibrations.

Tue Nov 17 00:00:00 UTC 2020

Mechanical suppression of breast cancer cell invasion and paracrine signaling to osteoclasts requires nucleo-cytoskeletal connectivity.

Bone research

Yi X,Wright LE,Pagnotti GM,Uzer G,Powell KM,Wallace JM,Sankar U,Rubin CT,Mohammad K,Guise TA,Thompson WR

MA5-18077 was used in Immunocytochemistry-immunoflourescence to examine the mechanisms by which cancer cells sense and respond to low-magnitude mechanical signals introduced from vibrations.

Tue Nov 17 00:00:00 UTC 2020

Spatiotemporal Mislocalization of Nuclear Membrane-Associated Proteins in γ-Irradiation-Induced Senescent Cells.

Cells

Svobodová Kovaříková A,Bártová E,Kovařík A,Lukášová E

MA5-18077 was used in Immunocytochemistry-immunoflourescence to show cellular senescence can be characterized phenotypically by mislocalization of linker of nucleoskeleton and cytoskeleton complex proteins.

Fri Apr 17 00:00:00 UTC 2020

Spatiotemporal Mislocalization of Nuclear Membrane-Associated Proteins in γ-Irradiation-Induced Senescent Cells.

Cells

Svobodová Kovaříková A,Bártová E,Kovařík A,Lukášová E

Fri Apr 17 00:00:00 UTC 2020

Spatiotemporal Mislocalization of Nuclear Membrane-Associated Proteins in γ-Irradiation-Induced Senescent Cells.

Cells

Svobodová Kovaříková A,Bártová E,Kovařík A,Lukášová E

Fri Apr 17 00:00:00 UTC 2020

Nup93 regulates breast tumor growth by modulating cell proliferation and actin cytoskeleton remodeling.

Life science alliance

Bersini S,Lytle NK,Schulte R,Huang L,Wahl GM,Hetzer MW

Published figure using Nesprin 1 monoclonal antibody (Product # MA5-18077) in Immunocytochemistry

Wed Jan 01 00:00:00 UTC 2020

Role of the nuclear membrane protein Emerin in front-rear polarity of the nucleus.

Nature communications

Nastały P,Purushothaman D,Marchesi S,Poli A,Lendenmann T,Kidiyoor GR,Beznoussenko GV,Lavore S,Romano OM,Poulikakos D,Lagomarsino MC,Mironov AA,Ferrari A,Maiuri P

MA5-18077 was used in Immunomicroscopy to demonstrate that front-rear cell polarity is transmitted to the nucleus and that emerin is an important determinant of nuclear polarity.

Fri May 01 00:00:00 UTC 2020

Role of the nuclear membrane protein Emerin in front-rear polarity of the nucleus.

Nature communications

Nastały P,Purushothaman D,Marchesi S,Poli A,Lendenmann T,Kidiyoor GR,Beznoussenko GV,Lavore S,Romano OM,Poulikakos D,Lagomarsino MC,Mironov AA,Ferrari A,Maiuri P

MA5-18077 was used in Immunomicroscopy to demonstrate that front-rear cell polarity is transmitted to the nucleus and that emerin is an important determinant of nuclear polarity.

Fri May 01 00:00:00 UTC 2020

Nuclear envelope rupture is induced by actin-based nucleus confinement.

The Journal of cell biology

Hatch EM,Hetzer MW

MA518077 was used in immunocytochemistry to demonstrate that nuclear envelope rupture in cancer cells relies on the assembly of contractile actin bundles that interact with the nucleus via the linker of nucleoskeleton and cytoskeleton complex

Mon Oct 10 00:00:00 UTC 2016

生物信息学

蛋白别名: CPG2 full length; DKFZp781J13156; Enaptin; enaptin165; FLJ30878; FLJ41140; KASH domain-containing protein 1; KASH1; Myne-1; Myocyte nuclear envelope protein 1; nesprin 1; Nesprin-1; Nuclear envelope spectrin repeat protein 1; RP1-130E4.2; spectrin repeat containing, nuclear envelope 1; synaptic nuclear envelope 1; Synaptic nuclear envelope protein 1; synaptic nuclei expressed gene 1; SYNE 1; Syne-1; syne-1 fragment

基因别名: 8B; A330049M09Rik; ARCA1; BE692247; C130039F11Rik; C6orf98; CPG2; dJ45H2.2; EDMD4; KIAA0796; KIAA1262; KIAA1756; mKIAA1756; Myne-1; MYNE1; Nesp1; SCAR8; SYNE1

UniProt ID: (Human) H0Y4C0, (Mouse) Q6ZWR6

Entrez Gene ID: (Human) 23345, (Mouse) 64009, (Rat) 499010

功能

actin binding protein binding lamin binding protein homodimerization activity poly(A) RNA binding actin filament binding receptor binding enzyme binding structural molecule activity

参与通路

nucleus organization Golgi organization establishment of nucleus localization muscle cell differentiation cytoskeletal anchoring at nuclear membrane nuclear matrix anchoring at nuclear membrane positive regulation of receptor-mediated endocytosis regulation of dendrite morphogenesis brain development response to light stimulus

Nesprin 1 Monoclonal Antibody (MANNES1A(7A12))

Nesprin 1 Antibody (MA5-18077) in ICC/IF

产品信息

MA5-18077

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

免疫电镜技术 (IM)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

Nesprin 1 Monoclonal Antibody (MANNES1A(7A12))

Nesprin 1 Antibody (MA5-18077) in ICC/IF

产品信息

MA5-18077

免疫印迹 (WB)

免疫细胞化学 (ICC/IF)

免疫电镜技术 (IM)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID .st0{fill-rule:evenodd;clip-rule:evenodd;fill:#A2A2A2;}

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

RRID