Alomone Labs, LTD

Neurofascin Polyclonal Antibody

Neurofascin Antibody in Immunohistochemistry (Frozen) (IHC (F))

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Neurofascin Antibody (AIP-025-200UL) in IHC (F)

Expression ofNeurofascinin mousehippocampus. Immunohistochemicalstaining of perfusion-fixed frozenmousebrain sections with Anti-Neurofascin Antibody (#AIP-025), (1:200), followed by goat Anti-rabbit-AlexaFluor-488.A.Staining in thehippocampal dentate gyrus region, showedNeurofascinimmunoreactivity (green)in thehilus (H) andinan outside layer (arrows).B. Pre-incubation of the Antibody with NeurofascinBlocki... View More

Neurofascin Antibody in Western Blot (WB)

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产品信息

AIP-025-200UL

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:800

免疫组化（冰冻） (IHC (F))

1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

(C)REKKDVPLGPEDPK, corresponding to amino acid residues 1142-1155 of rat NFASC, Intracellular, C-terminus

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.8 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4, with 1% BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

靶标信息

Neurofascin encodes an L1 family immunoglobulin cell adhesion molecule with multiple IGcam and fibronectin domains. The protein functions in neurite outgrowth, neurite fasciculation, and organization of the axon initial segment (AIS) and nodes of Ranvier on axons during early development. Both the AIS and nodes of Ranvier contain high densities of voltage-gated Na+ (Nav) channels which are clustered by interactions with cytoskeletal and scaffolding proteins including this protein, gliomedin, ankyrin 3 (ankyrin-G), and betaIV spectrin. This protein links the AIS extracellular matrix to the intracellular cytoskeleton. Neurofascin undergoes extensive alternative splicing, and the full-length nature of some variants has not been determined.

仅用于科研。不用于诊断过程。未经明确授权不得转售。