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Application
FIGURE: 1 / 1
Neurturin Antibody (500-P102BT-25UG) in ELISA
Sandwich ELISA: To detect Human Neurturin by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of Neurturin Polyclonal Antibody, Biotin (Product # 500-P102BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Neurturin Polyclonal Antibody (Product # 500-P102-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Neurturin.
Product Details
500-P102BT-25UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived, 23.6 kDa Recombinant Human Neurturin
Conjugate
Biotin
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929195
Product Specific Information
AA Sequence of recombinant protein: ARLGARPCGL RELEVRVSEL GLGYASDETV LFRYCAGACE AAARVYDLGL RRLRQRRRLR RERVRAQPCC RPTAYEDEVS FLDAHSRYHT VHELSARECA CV.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Neurturin. Anti-Human Neurturin-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect Human Neurturin by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human Neurturin (500-P102) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Neurturin.
Western Blot: To detect hNeurturin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hNeurturin is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P102BT-1MG will be provided as 2 x 500 µg
Target Information
Glial cell line-derived neurotrophic factor (GDNF) plays key roles in the control of vertebrate neuron survival and differentiation. A novel neurotrophic factor was recently cloned from human and mouse and designated neurturin. Physiological responses to neurturin (NTN) require the presence of receptor tyrosine kinase RET and a novel glycosylphosphatidylinositol linked receptor NTNRalpha. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned recently and termed GDNFR-b, Ret ligand 2 (RETL2) or TGF-beta-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFR-a-2 recently. NTN binds to and forms a complex with GFR-a-2 and the Ret PTK and activates the RET receptor tyrosine kinase pathway. Both NTN and GDNF can activate the MAP kinase and phosphatidylinositol 3-kinase pathways and play a critical role in the development of many neuronal populations. Neurturin and GDNF define a new family of neurotrophic factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Neurturin; prepro-neurturin
Gene Aliases: NRTN; NTN
UniProt ID: (Human) Q99748
Entrez Gene ID: (Human) 4902
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