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Ninein Antibody (PA5-96974) in IHC (P)
Immunohistochemistry analysis of Ninein in paraffin-embedded human stomach. Samples were incubated with Ninein Polyclonal antibody (Product # PA5-96974) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
产品信息
PA5-96974
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of human NIN (NP_8919911)
偶联物
形式
Liquid
浓度
1.21 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2808776
产品详细信息
Immunogen sequence: MDEVEQDQHE ARLKELFDSF DTTGTGSLGQ EELTDLCHML SLEEVAPVLQ QTLLQDNLLG RVHFDQFKEA LILILSRTLS NEEHFQEPDC SLEAQPKYVR GGKRYGRRSL PEFQESVEEF PEVTVIEPLD EEARPSHIPA GDCSEHWKTQ RSEEYEAEGQ LRFWNPDDLN ASQSGSSPPQ DWIEEKLQEV CEDLGITRDG HLNRKKLVSI CEQYGLQNVD GEMLEEVFHN LDPDGTMSVE DFFYGLFKNG KSLTPSASTP YRQLKRHLSM QSFDESGRRT TTSSAMT; Positive Samples: Jurkat; Cellular Location: Cytoplasm, centrosome, cytoskeleton, microtubule organizing center
靶标信息
Ninein is a component of subdistal appendages of the mother centriole, localized specifically in the pericentriolar matrix of the centrosome. It may be involved in microtubule minus-end capping, centriole positioning, and protein anchoring. Recent studies have shown that the microtubule nucleation and anchoring at the centrosome are independent processes linked by ninein function. At least five human ninein isoforms with divergent C-terminal domains and two mouse isoforms have been reported. Data indicates that the combined action of domains (C-terminal and N-terminal) might, in the absence of coiled coil region, be sufficient to localize ninein to the mother centriole. Microinjection of antibodies against ninein into metaphase HeLa cells can disrupt the reformation of tubular conformation of proteins within the centrosome following cell division, and consequently lead to dispersal of centrosomal material throughout the cytosol. MTOC (microtubule organizing center) function can be disrupted when anti-ninein antibodies are injected into postmitotic cells. Antibodies against ninein are present in sera from patients with autoimmune diseases that develop autoantibodies to centrosomal proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Glycogen synthase kinase 3 beta-interacting protein; GSK3B-interacting protein; hNinein; Ninein; ninein (GSK3B interacting protein); ninein centrosomal protein
基因别名: 3110068G20Rik; AI385615; AU024711; KIAA1565; mKIAA1565; NIN; SCKL7
UniProt ID: (Human) Q8N4C6, (Mouse) Q61043
Entrez Gene ID: (Human) 51199, (Mouse) 18080, (Rat) 299117
