Invitrogen

Nkx2.5 Polyclonal Antibody

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

7个已发表图片

11 篇参考文献

查看其他20个Nkx2.5抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Nkx2.5 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 14

Nkx2.5 Antibody (PA5-49431) in ICC/IF

Immunocytochemistry analysis of Nkx2.5 in C2C12 cells. Samples were incubated with Nkx2.5 polyclonal antibody (Product # PA5-49431) using a dilution of 1:25 followed by Dylight® 488-conjugated goat anti-Rabbit IgG at a dilution of 1:200 (green). Cells were 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized. Immunofluorescence image showing Nucleus staining on C2C12 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (red). The nuclear counter stain is DAPI (blue).

Nkx2.5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Nkx2.5 Antibody in Flow Cytometry (Flow)

Nkx2.5 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

产品信息

PA5-49431

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:500

免疫细胞化学 (ICC/IF)

1:25

查看3篇已发表文章 3篇已发表文章

流式细胞分析 (Flow)

1:25

查看2篇已发表文章 2篇已发表文章

染色质免疫沉淀实验 (ChIP)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse, Zebrafish

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

KLH conjugated synthetic peptide between 98-133 amino acids from the Central region of mouse Nkx2-5.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A, Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2634885

靶标信息

This gene encodes a homeobox-containing transcription factor. This transcription factor functions in heart formation and development. Mutations in this gene cause atrial septal defect with atrioventricular conduction defect, and also tetralogy of Fallot, which are both heart malformation diseases. Mutations in this gene can also cause congenital hypothyroidism non-goitrous type 5, a non-autoimmune condition. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Reprogramming of dermal fibroblasts from a Duchenne muscular dystrophy patient carrying a deletion of exons 45-50 into an induced pluripotent stem cell line (CCMi005-A).

Stem cell research

Rovina D,Castiglioni E,Mallia S,Rabino M,Farini A,Belicchi M,Di Giuseppe G,Gervasini C,Torrente Y,Pompilio G,Gowran A

PA5-49431 was used in Flow cytometry/Cell sorting to show that the established iPSC line exhibited normal morphology, expressed pluripotency markers, had normal karyotype and possessed trilineage differentiation potential.

Sat Oct 01 00:00:00 EDT 2022

Reprogramming of dermal fibroblasts from a Duchenne muscular dystrophy patient carrying a deletion of exons 45-50 into an induced pluripotent stem cell line (CCMi005-A).

Stem cell research

Rovina D,Castiglioni E,Mallia S,Rabino M,Farini A,Belicchi M,Di Giuseppe G,Gervasini C,Torrente Y,Pompilio G,Gowran A

Sat Oct 01 00:00:00 EDT 2022

In situ differentiation of human-induced pluripotent stem cells into functional cardiomyocytes on a coaxial PCL-gelatin nanofibrous scaffold.

Materials science & engineering. C, Materials for biological applications

Sridharan D,Palaniappan A,Blackstone BN,Dougherty JA,Kumar N,Seshagiri PB,Sayed N,Powell HM,Khan M

PA5-49431 was used in Immunocytochemistry to demonstrate that coaxial polycaprolactone-gelatin nanofibrous scaffolds can be used as a 3D culture platform for efficient differentiation of human-induced pluripotent stem cells to functional cardiomyocytes.

Fri Jan 01 00:00:00 EST 2021

Human Cardiac Progenitor Cells Enhance Exosome Release and Promote Angiogenesis Under Physoxia.

Frontiers in cell and developmental biology

Dougherty JA,Patel N,Kumar N,Rao SG,Angelos MG,Singh H,Cai C,Khan M

Published figure using Nkx2.5 polyclonal antibody (Product # PA5-49431) in Immunocytochemistry

Mon Sep 28 00:00:00 EDT 2020

Fetal Hypoxia Impacts on Proliferation and Differentiation of Sca-1⁺ Cardiac Progenitor Cells and Maturation of Cardiomyocytes: A Role of MicroRNA-210.

Genes

Meng X,Zhang P,Zhang L

PA5-49431 was used in Flow Cytometry to examine the effect of hypoxic stress on the regulations of CPC fate decisions and the potential mechanism.

Fri Mar 20 00:00:00 EDT 2020

Fetal Hypoxia Impacts on Proliferation and Differentiation of Sca-1⁺ Cardiac Progenitor Cells and Maturation of Cardiomyocytes: A Role of MicroRNA-210.

Genes

Meng X,Zhang P,Zhang L

PA5-49431 was used in Flow Cytometry to examine the effect of hypoxic stress on the regulations of CPC fate decisions and the potential mechanism.

Fri Mar 20 00:00:00 EDT 2020

Activating transcription factor 3 coordinates differentiation of cardiac and hematopoietic progenitors by regulating glucose metabolism.

Science advances

Yin HM,Yan LF,Liu Q,Peng Z,Zhang CY,Xia Y,Su D,Gu AH,Zhou Y

Published figure using Nkx2.5 polyclonal antibody (Product # PA5-49431) in ChIP assay

Fri May 01 00:00:00 EDT 2020

Activating transcription factor 3 coordinates differentiation of cardiac and hematopoietic progenitors by regulating glucose metabolism.

Science advances

Yin HM,Yan LF,Liu Q,Peng Z,Zhang CY,Xia Y,Su D,Gu AH,Zhou Y

Published figure using Nkx2.5 polyclonal antibody (Product # PA5-49431) in ChIP assay

Fri May 01 00:00:00 EDT 2020

Intercalated cushion cells within the cardiac outflow tract are derived from the myocardial troponin T type 2 (Tnnt2) Cre lineage.

Developmental dynamics : an official publication of the American Association of Anatomists

Mifflin JJ,Dupuis LE,Alcala NE,Russell LG,Kern CB

PA5-49431 was used in Immunohistochemistry to generate three-dimensional lineage mapping of AV and PV cusps during intercalated cushion development.

Wed Aug 01 00:00:00 EDT 2018

Single cell RNA-seq and ATAC-seq analysis of cardiac progenitor cell transition states and lineage settlement.

Nature communications

Jia G,Preussner J,Chen X,Guenther S,Yuan X,Yekelchyk M,Kuenne C,Looso M,Zhou Y,Teichmann S,Braun T

PA5-49431 was used in Immunohistochemistry to characterise mouse cardiac progenitor cells marked by Nkx2-5 and Isl1 expression from E7.5 to E9.5.

Mon Nov 19 00:00:00 EST 2018

The pro-inflammatory signalling regulator Stat4 promotes vasculogenesis of great vessels derived from endothelial precursors.

Nature communications

Meng ZZ,Liu W,Xia Y,Yin HM,Zhang CY,Su D,Yan LF,Gu AH,Zhou Y

Published figure using Nkx2.5 polyclonal antibody (Product # PA5-49431) in Immunohistochemistry

Fri Mar 03 00:00:00 EST 2017

The pro-inflammatory signalling regulator Stat4 promotes vasculogenesis of great vessels derived from endothelial precursors.

Nature communications

Meng ZZ,Liu W,Xia Y,Yin HM,Zhang CY,Su D,Yan LF,Gu AH,Zhou Y

Published figure using Nkx2.5 polyclonal antibody (Product # PA5-49431) in Immunohistochemistry

Fri Mar 03 00:00:00 EST 2017

The pro-inflammatory signalling regulator Stat4 promotes vasculogenesis of great vessels derived from endothelial precursors.

Nature communications

Meng ZZ,Liu W,Xia Y,Yin HM,Zhang CY,Su D,Yan LF,Gu AH,Zhou Y

Published figure using Nkx2.5 polyclonal antibody (Product # PA5-49431) in Immunohistochemistry

Fri Mar 03 00:00:00 EST 2017

生物信息学

蛋白别名: Cardiac-specific homeobox; cardiac-specific homeobox 1; Drosophila NK2 transcription factor related, locus 5; FLJ52202; FLJ97166; FLJ97195; FLJ97197; FLJ99536; Homeobox protein CSX; Homeobox protein NK-2 homolog E; homeobox protein NKX 2-5; Homeobox protein Nkx-2.5; NK2 transcription factor related locus 5; NK2 transcription factor related, locus 5; NKX 2-5; Nkx-25; tinman paralog

查看更多收起

基因别名: CHNG5; CSX; CSX1; HLHS2; Nkx-2.5; NKX2-5; NKX2.5; NKX2E; NKX4-1; tinman; VSD3

查看更多收起

UniProt ID: (Human) P52952, (Mouse) P42582

查看更多收起

Entrez Gene ID: (Human) 1482, (Mouse) 18091

查看更多收起

功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription cofactor activity transcriptional activator activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding protein binding transcription factor binding serum response element binding protein homodimerization activity sequence-specific DNA binding transcription regulatory region DNA binding protein heterodimerization activity histone deacetylase binding homeodomain transcription factor

参与通路

Nkx2.5 Polyclonal Antibody

Nkx2.5 Antibody (PA5-49431) in ICC/IF

产品信息

PA5-49431

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

Nkx2.5 Polyclonal Antibody

Nkx2.5 Antibody (PA5-49431) in ICC/IF

产品信息

PA5-49431

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)