Invitrogen

Nrf2 Polyclonal Antibody

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

5个已发表图片

8 篇参考文献

查看其他27个Nrf2抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Nrf2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 16

Nrf2 Antibody (PA5-88084) in ICC/IF

Immunofluorescence analysis of Nrf2 in HeLa cells. Samples were incubated with Nrf2 Polyclonal antibody (Product # PA5-88084) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Nrf2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

Nrf2 Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using Nrf2 Polyclonal Antibody (Product # PA5-88084), shows the upregulation of Nrf2 expression upon treatment with MG-132 and TBHQ in U-2 OS, MDA-MB-231, Hep G2 and HEK-293 cell lines. {TM}

产品信息

PA5-88084

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

查看6篇已发表文章 6篇已发表文章

免疫组化 (IHC)

查看1篇已发表文章 1篇已发表文章

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

查看1篇已发表文章 1篇已发表文章

酶联免疫吸附实验 (ELISA)

1 µg/mL

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence within amino acids 366-605 of human NRF2 (NP_0061552)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.92 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2804638

产品详细信息

Positive Samples: A-549, HepG2, HeLa, Rat liver; Cellular Location: Cytoplasm, Nucleus, cytosol

Immunogen sequence: YGDTLLGLSD SEVEELDSAP GSVKQNGPKT PVHSSGDMVQ PLSPSQGQST HVHDAQCENT PEKELPVSPG HRKTPFTKDK HSSRLEAHLT RDELRAKALH IPFPVEKIIN LPVVDFNEMM SKEQFNEAQL ALIRDIRRRG KNKVAAQNCR KRKLENIVEL EQDLDHLKDE KEKLLKEKGE NDKSLHLLKK QLSTLYLEVF SMLRDEDGKP YSPSEYSLQQ TRDGNVFLVP KSKKPDVKKN

靶标信息

Nfr1 (also designated TCF11), Nrf2 and Nfr3 are bZIP transcription factors that heterodimer with Maf proteins to bind MARE (Maf recognition elements) sequences. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The cardioprotective effects of secoisolariciresinol diglucoside (flaxseed lignan) against cafeteria diet-induced cardiac fibrosis and vascular injury in rats: an insight into apelin/AMPK/FOXO3a signaling pathways.

Frontiers in pharmacology

Abdelwahab AH,Negm AM,Mahmoud ES,Salama RM,Schaalan MF,El-Sheikh AAK,Ramadan BK

Published figure using Nrf2 polyclonal antibody (Product # PA5-88084) in Western Blot

Fri Jul 28 00:00:00 EDT 2023

Gut microbiota-derived tryptophan metabolites alleviate liver injury via AhR/Nrf2 activation in pyrrolizidine alkaloids-induced sinusoidal obstruction syndrome.

Cell & bioscience

Shang H,Huang C,Xiao Z,Yang P,Zhang S,Hou X,Zhang L

PA5-88084 was used in Western Blotting to investigate the role of gut microbiota in hepatic sinusoidal obstruction syndrome (HSOS), highlighting the impact of microbial tryptophan metabolism and the AhR/Nrf2 signaling pathway in MCT-induced liver injury.

Sat Jul 08 00:00:00 EDT 2023

Flavonoid-Conjugated Gadolinium Complexes as Anti-Inflammatory Theranostic Agents.

Antioxidants (Basel, Switzerland)

Yang BW,Yang S,Kim S,Baek AR,Sung B,Kim YH,Lee JT,Lee SY,Kim HK,Choi G,Park JA,Nam SW,Lee GH,Chang Y

Published figure using Nrf2 polyclonal antibody (Product # PA5-88084) in Western Blot

Thu Dec 15 00:00:00 EST 2022

Galangin attenuates cadmium-evoked nephrotoxicity: Targeting nucleotide-binding domain-like receptor pyrin domain containing 3 inflammasome, nuclear factor erythroid 2-related factor 2, and nuclear factor kappa B signaling.

Journal of biochemical and molecular toxicology

Salama SA,Abd-Allah GM,Gad HS,Kabel AM

Published figure using Nrf2 polyclonal antibody (Product # PA5-88084) in Western Blot

Fri Jul 01 00:00:00 EDT 2022

Journal of biochemical and molecular toxicology

Salama SA,Abd-Allah GM,Gad HS,Kabel AM

Published figure using Nrf2 polyclonal antibody (Product # PA5-88084) in Western Blot

Fri Jul 01 00:00:00 EDT 2022

Short-term exposure to JUUL electronic cigarettes can worsen ischemic stroke outcome.

Fluids and barriers of the CNS

Sifat AE,Archie SR,Nozohouri S,Villalba H,Zhang Y,Sharma S,Ghanwatkar Y,Vaidya B,Mara D,Cucullo L,Abbruscato TJ

Published figure using Nrf2 polyclonal antibody (Product # PA5-88084) in Western Blot

Fri Sep 09 00:00:00 EDT 2022

Short-term exposure to JUUL electronic cigarettes can worsen ischemic stroke outcome.

Fluids and barriers of the CNS

Sifat AE,Archie SR,Nozohouri S,Villalba H,Zhang Y,Sharma S,Ghanwatkar Y,Vaidya B,Mara D,Cucullo L,Abbruscato TJ

Published figure using Nrf2 polyclonal antibody (Product # PA5-88084) in Western Blot

Fri Sep 09 00:00:00 EDT 2022

circ_0072464 Shuttled by Bone Mesenchymal Stem Cell-Secreted Extracellular Vesicles Inhibits Nucleus Pulposus Cell Ferroptosis to Relieve Intervertebral Disc Degeneration.

Oxidative medicine and cellular longevity

Yu X,Xu H,Liu Q,Wang Y,Wang S,Lu R,Jiang Y,Kang H,Hu W

PA5-88084 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that BMSC-EV-loaded circ_0072464 inhibited NPC ferroptosis to relieve IDD via upregulation of miR-431-mediated NRF2, therefore providing a potential therapeutic target against IDD.

Tue Jul 12 00:00:00 EDT 2022

Impact of chronic smoking on traumatic brain microvascular injury: An in vitro study.

Journal of cellular and molecular medicine

Sivandzade F,Alqahtani F,Cucullo L

PA5-88084 was used in Western Blot to show that chronic TS exposure exacerbates traumatic endothelial injury and abrogates the protective antioxidative cell responses.

Sun Aug 01 00:00:00 EDT 2021

Lysosomal nitric oxide determines transition from autophagy to ferroptosis after exposure to plasma-activated Ringer's lactate.

Redox biology

Jiang L,Zheng H,Lyu Q,Hayashi S,Sato K,Sekido Y,Nakamura K,Tanaka H,Ishikawa K,Kajiyama H,Mizuno M,Hori M,Toyokuni S

Published figure using Nrf2 polyclonal antibody (Product # PA5-88084) in Immunocytochemistry

Thu Jul 01 00:00:00 EDT 2021

Lysosomal nitric oxide determines transition from autophagy to ferroptosis after exposure to plasma-activated Ringer's lactate.

Redox biology

Jiang L,Zheng H,Lyu Q,Hayashi S,Sato K,Sekido Y,Nakamura K,Tanaka H,Ishikawa K,Kajiyama H,Mizuno M,Hori M,Toyokuni S

Published figure using Nrf2 polyclonal antibody (Product # PA5-88084) in Immunocytochemistry

Thu Jul 01 00:00:00 EDT 2021

生物信息学

蛋白别名: E4TF1-60; E4TF1A; NF-E2-related factor 2; NFE2-related factor 2; NFT2; nrf2; NRF2A; nuclear factor erythroid 2-like 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2; nuclear factor, erythroid 2-like 2; Nuclear factor, erythroid derived 2, like 2

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基因别名: AI194320; HEBP1; NFE2L2; NRF2

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UniProt ID: (Human) Q16236, (Mouse) Q60795, (Rat) O54968

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Entrez Gene ID: (Human) 4780, (Mouse) 18024, (Rat) 83619

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