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Invitrogen
NuMA Polyclonal Antibody
高级验证
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
图: 1 / 10
NuMA Antibody (PA5-22285) in ICC/IF
Immunofluorescence analysis of NuMA was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with NuMA Polyclonal Antibody (Product # PA5-22285) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1... View More
产品信息
PA5-22285
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fragment corresponding to a region within amino acids 1 and 339 of NuMA (Uniprot ID#Q14980)
偶联物
形式
Liquid
浓度
0.54 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_11157241
产品详细信息
Recommended positive controls: 293T, A431, HeLa, HepG2, HeLa, HeLa (100 ng/ml Nocodazole treatment for 24 hr).
Predicted reactivity: Mouse (81%), Rat (83%), Bovine (89%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
NuMA (nuclear mitotic apparatus) is a long coil-coiled protein that plays a role in the interphase and mitosis phases of a cell cycle. In interphase, it has been hypothesized to play a structural role in the nucleoskeleton that may be important in nuclear organization and functions. During mitosis, it is associated with spindle microtubule organization and chromosome positioning.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: centrophilin stabilizes mitotic spindle in mitotic cells; NMP-22; Nuclear matrix protein-22; Nuclear mitotic apparatus protein; Nuclear mitotic apparatus protein 1; NuMA protein; SP-H antigen; structural nuclear protein
基因别名: NMP-22; NMP22; NUMA; NUMA1
UniProt ID: (Human) Q14980
Entrez Gene ID: (Human) 4926
