Invitrogen

Nucleolin Recombinant Rabbit Monoclonal Antibody (JE65-71)

Nucleolin Antibody in Immunocytochemistry (ICC/IF)

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Nucleolin Antibody (MA5-44735) in ICC/IF

Immunocytochemistry analysis of Nucleolin in HeLa cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 , permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Samples were incubated in Nucleolin Monoclonal antibody (Product # MA5-44735) using a dilution of 1:200 in 2% negative goat serum overnight at 4 fo... View More

Nucleolin Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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产品信息

MA5-44735

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

免疫组化（石蜡） (IHC (P))

1:200-1:400

免疫细胞化学 (ICC/IF)

1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JE65-71

抗原

Synthetic peptide within human CSDE1 aa 661-710/710.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 0.05% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2931193

靶标信息

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

仅用于科研。不用于诊断过程。未经明确授权不得转售。