NeoBiotechnologies

Nucleoside Diphosphate Kinase A/nm23-H1 Monoclonal Antibody (NME1/2737)

Nucleoside Diphosphate Kinase A/nm23-H1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Nucleoside Diphosphate Kinase A/nm23-H1 Antibody (4830-MSM7-P1) in IHC (P)

Formalin-fixed, paraffin-embedded human breast carcinoma stained with NME1/nm23-H1 Mouse Monoclonal Antibody (NME1/2737).

Nucleoside Diphosphate Kinase A/nm23-H1 Antibody in Western Blot (WB)

Nucleoside Diphosphate Kinase A/nm23-H1 Antibody in Peptide array (ARRAY)

Nucleoside Diphosphate Kinase A/nm23-H1 Antibody in Immunoelectrophoresis (IE)

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产品信息

4830-MSM7-P1

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

免疫组化（石蜡） (IHC (P))

1-2 µg/mL

酶联免疫吸附实验 (ELISA)

Assay-dependent

肽阵列 (Array)

Assay-dependent

免疫电泳 (IE)

Assay-dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

NME1/2737

抗原

Recombinant full-length human NME1 protein

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

200 µg/mL

纯化类型

Protein A/G

保存液

PBS with 0.05% BSA

内含物

0.05% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Antibody is stable for 24 months.^M
^M
Positive Control: HeLa, A549 or Jurkat cells. Ubiquitously expressed in all tissues. Cellular Localization: Cytoplasm. Nucleus. Cell-cycle dependent nuclear localization.^M
^M
Specificity Comments: The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sub-lines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nuclease hypersensitive elements at positions 142 to 115 of the human c-Myc promotor.

靶标信息

NME1 was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in the gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: GAAD; Granzyme A activated DNase (GAAD); Granzyme A-activated DNase; GZMA activated DNase; included; Metastasis inhibition factor nm23; NDK A; NDP kinase A; NM23-H1; NM23H1B; NME1-NME2 spliced read-through transcript; non-metastatic cells 1, protein (NM23A) expressed in; Nucleoside diphosphate kinase A; Tumor metastatic process-associated protein

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基因别名: AWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1; NME1

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UniProt ID: (Human) P15531

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Entrez Gene ID: (Human) 4830

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