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OAT Antibody (PA5-35370) in ICC/IF
Immunocytochemistry analysis of OAT in A549 cells. Samples were incubated with OAT polyclonal antibody (Product # PA5-35370) using a dilution of 1:25 for 1 h at 37°C followed by Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) at a dilution of 1:400 for 50 min at 37°C. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.1%, 10 min). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/mL, 1 h at 37°C). OAT immunoreactivity is localized to Mitochondrion signi... View More
产品信息
PA5-35370
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
KLH conjugated synthetic peptide between 27-55 amino acids from the N-terminal region of human OAT
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A, Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2552680
靶标信息
OAT is the mitochondrial enzyme ornithine aminotransferase, which is a key enzyme in the pathway that converts arginine and ornithine into the major excitatory and inhibitory neurotransmitters glutamate and GABA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: gyrate atrophy; ornithine aminotransferase (gyrate atrophy); Ornithine aminotransferase, mitochondrial; Ornithine delta-aminotransferase; Ornithine--oxo-acid aminotransferase; ornithine-delta-aminotransferase; ornithine-oxo-acid aminotransferase; testicular tissue protein Li 130
基因别名: AI194874; GACR; HOGA; OAT; OATASE; OKT; rOAT
UniProt ID: (Human) P04181, (Mouse) P29758, (Rat) P04182
Entrez Gene ID: (Human) 4942, (Mouse) 18242, (Rat) 64313
