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Invitrogen
OCT1 (POU2F1) Monoclonal Antibody (YL15), eBioscience™
高级验证
This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.
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OCT1 (POU2F1) Antibody (14-9736-82) in ICC/IF
Immunofluorescent analysis of POU2F1 (green) in A549 cells (60 % confluency). The cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at 37 C, and blocked with 10% Normal Donkey Serum in PBS for 1 hour at room temperature. Cells were then stained with OCT1 (POU2F1) Monoclonal Antibody, Unconjugated at 1.25 µg/mL Mouse IgG1 isotype... View More
产品信息
14-9736-82
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
YL15
抗原
Recombinant full-length human OCT1 (POU2F1)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4° C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2802196
产品详细信息
Description: This YL15 monoclonal antibody recognizes human OCT1 (POU2F1) and is predicted to recognize mouse POU2F1.
Applications Reported: This YL15 monoclonal antibody has been reported for use in western blot and immunocytochemical staining.
Applications Tested: This YL15 antibody has been tested by western blot on HeLa wild type and POU2F1 knock-out lysate and NTERA-2 lysate. This may be used at less than or equal to 4 µg/mL. This YL15 antibody has been tested by immunohistochemical staining of cultured A549 cells. This may be used at less than or equal to 1.25 µg/mL.
靶标信息
OCT1 (POU2F1) is a ubiquitously expressed transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. It modulates transcription transactivation by NR3C1, AR and PGR. In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: NF-A1; Oct-1; Octamer-binding protein 1; Octamer-binding transcription factor 1; Octamer-binding transcription factor-1; OTF-1; POU domain, class 2, transcription factor 1
基因别名: oct-1B; OCT1; OTF1; POU2F1
UniProt ID: (Human) P14859
Entrez Gene ID: (Human) 5451
