Invitrogen

OCT4 Polyclonal Antibody

OCT4 Antibody in Immunocytochemistry (ICC/IF)

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OCT4 Antibody (PA5-27438) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of OCT4 was performed in NT2D1 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: OCT4 Polyclonal Antibody (Product # PA5-27438) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

OCT4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

OCT4 Antibody in Immunoprecipitation (IP)

OCT4 Antibody in Immunohistochemistry (IHC)

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产品信息

PA5-27438

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000-1:100,000

查看3篇已发表文章 3篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

查看1篇已发表文章 1篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:100-1:1,000

查看9篇已发表文章 9篇已发表文章

流式细胞分析 (Flow)

1:50-1:200

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

1:500-1:1,000

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 360 of Human OCT3/4

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2544914

产品详细信息

Recommended positive controls: human ESC, mouse ESC, NT2D1.

Predicted reactivity: Mouse (83%), Rat (83%), Cat (94%), Pig (93%), Rabbit (90%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (91%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

POU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Correction of DMD in human iPSC-derived cardiomyocytes by base-editing-induced exon skipping.

Molecular therapy. Methods & clinical development

Wang P,Li H,Zhu M,Han RY,Guo S,Han R

PA5-27438 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to explore the feasibility of base-editing-mediated exon skipping as a therapeutic strategy for DMD using cardiomyocytes derived from human induced pluripotent stem cells (hiPSCs).

Thu Mar 09 00:00:00 EST 2023

Correction of DMD in human iPSC-derived cardiomyocytes by base-editing-induced exon skipping.

Molecular therapy. Methods & clinical development

Wang P,Li H,Zhu M,Han RY,Guo S,Han R

Thu Mar 09 00:00:00 EST 2023

Differentiation-dependent changes in lamin B1 dynamics and lamin B receptor localization.

Molecular biology of the cell

Wesley CC,Levy DL

PA5-27438 was used in Immunohistochemistry and Western Blotting to suggest that differentiation-dependent tethering of lamin B1 either directly by LBR or indirectly via LBR-chromatin associations impacts gene expression.

Wed Feb 01 00:00:00 EST 2023

Differentiation-dependent changes in lamin B1 dynamics and lamin B receptor localization.

Molecular biology of the cell

Wesley CC,Levy DL

Wed Feb 01 00:00:00 EST 2023

Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.

Neuron

LaForce GR,Farr JS,Liu J,Akesson C,Gumus E,Pinkard O,Miranda HC,Johnson K,Sweet TJ,Ji P,Lin A,Coller J,Philippidou P,Wagner EJ,Schaffer AE

PA5-27438 was used in Immunocytochemistry-immunoflourescence to identify a previously uncharacterized role for CLP1 in mRNA 3' end formation and reveal an mRNA misprocessing signature in neurodegeneration that may suggest a common mechanism of disease.

Wed Apr 20 00:00:00 EDT 2022

Patient-specific, re-engineered cardiomyocyte model confirms the circumstance-dependent arrhythmia risk associated with the African-specific common SCN5A polymorphism p.S1103Y: Implications for the increased sudden deaths observed in black individuals during the COVID-19 pandemic.

Heart rhythm

Hamrick SK,John Kim CS,Tester DJ,Giudicessi JR,Ackerman MJ

PA5-27438 was used in Immunocytochemistry to ascertain the effects of acidosis and hydroxychloroquine (HCQ) on the action potential duration (APD) in a patient-specific induced pluripotent stem cell-derived cardiomyocyte (iPSC-CM) model of p.S1103Y-SCN5A.

Sun May 01 00:00:00 EDT 2022

Determining epigenetic memory in kidney proximal tubule cell derived induced pluripotent stem cells using a quadruple transgenic reprogrammable mouse.

Scientific reports

Khelifi G,Chow T,Whiteley J,Fort V,Humphreys BD,Hussein SMI,Rogers IM

PA5-27438 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to demonstrate that early passage PTC iPSCs and TTF iPSCs expressed low levels of renal progenitor genes and high levels of pluripotency-associated genes, and the transcriptional levels of these genes were not significantly different between PTC iPSCs and TTF iPSCs.

Fri Nov 25 00:00:00 EST 2022

Determining epigenetic memory in kidney proximal tubule cell derived induced pluripotent stem cells using a quadruple transgenic reprogrammable mouse.

Scientific reports

Khelifi G,Chow T,Whiteley J,Fort V,Humphreys BD,Hussein SMI,Rogers IM

Fri Nov 25 00:00:00 EST 2022

Establishment of the iPSC line CUIMCi005-A from a patient with Stargardt disease for retinal organoid culture.

Stem cell research

Su PY,Lee W,Zernant J,Tsang SH,Nagasaki T,Corneo B,Allikmets R

PA5-27438 was used in Immunocytochemistry-immunofluorescence to establish an induced pluripotent stem cell line for retinal organoid research from a patient with mild disease features who is compound heterozygous for the frequent c.5882G>A (p.Gly1961Glu) missense variant and a c.4947delC (p.Glu1650Argfs*12) frameshift variant.

Thu Dec 01 00:00:00 EST 2022

Characterization of N-terminal RYR2 variants outside CPVT1 hotspot regions using patient iPSCs reveal pathogenesis and therapeutic potential.

Stem cell reports

Stutzman MJ,Kim CSJ,Tester DJ,Hamrick SK,Dotzler SM,Giudicessi JR,Miotto MC,Gc JB,Frank J,Marks AR,Ackerman MJ

PA5-27438 was used in Immunocytochemistry-immunoflourescence to show that these variants are located at the N-terminal domain and the central domain interface and could destabilize the RYR2 channel promoting Ca2+ leak-triggered arrhythmias.

Tue Sep 13 00:00:00 EDT 2022

In situ differentiation of human-induced pluripotent stem cells into functional cardiomyocytes on a coaxial PCL-gelatin nanofibrous scaffold.

Materials science & engineering. C, Materials for biological applications

Sridharan D,Palaniappan A,Blackstone BN,Dougherty JA,Kumar N,Seshagiri PB,Sayed N,Powell HM,Khan M

PA5-27438 was used in Immunocytochemistry to demonstrate that coaxial polycaprolactone-gelatin nanofibrous scaffolds can be used as a 3D culture platform for efficient differentiation of human-induced pluripotent stem cells to functional cardiomyocytes.

Fri Jan 01 00:00:00 EST 2021

Bone marrow-derived mesenchymal stem cells combined with gonadotropin therapy restore postnatal oogenesis of chemo-ablated ovaries in rats via enhancing very small embryonic-like stem cells.

Stem cell research & therapy

Ebrahim N,Al Saihati HA,Shaman A,Dessouky AA,Farid AS,Hussien NI,Mostafa O,Seleem Y,Sabry D,Saad AS,Emam HT,Hassouna A,Badr OAM,Saffaf BA,Forsyth NR,Salim RF

Published figure using OCT4 polyclonal antibody (Product # PA5-27438) in Immunohistochemistry

Mon Sep 27 00:00:00 EDT 2021

Cytosine and adenosine base editing in human pluripotent stem cells using transient reporters for editing enrichment.

Nature protocols

Tekel SJ,Brookhouser N,Standage-Beier K,Wang X,Brafman DA

PA5-27438 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot to facilitate the highly efficient single-base editing of human cells at precise genomic loci.

Thu Jul 01 00:00:00 EDT 2021

The master transcription factor SOX2, mutated in anophthalmia/microphthalmia, is post-transcriptionally regulated by the conserved RNA-binding protein RBM24 in vertebrate eye development.

Human molecular genetics

Dash S,Brastrom LK,Patel SD,Scott CA,Slusarski DC,Lachke SA

PA5-27438 was used in Immunocytochemistry to investigate whether the conserved RNA-binding motif protein, RBM24, positively controls Sox2 mRNA stability and is necessary for optimal SOX2 mRNA and protein levels in development, perturbation of which causes ocular defects, including microphthalmia and anophthalmia.

Fri Mar 13 00:00:00 EDT 2020

Meagre Argyrosomus regius (Asso, 1801) Stem Spermatogonia: Histological Characterization, Immunostaining, In Vitro Proliferation, and Cryopreservation.

Animals : an open access journal from MDPI

Zupa R,Martino NA,Marzano G,Dell'Aquila ME,Corriero A

Published figure using OCT4 polyclonal antibody (Product # PA5-27438) in Immunohistochemistry

Thu May 14 00:00:00 EDT 2020

Meagre Argyrosomus regius (Asso, 1801) Stem Spermatogonia: Histological Characterization, Immunostaining, In Vitro Proliferation, and Cryopreservation.

Animals : an open access journal from MDPI

Zupa R,Martino NA,Marzano G,Dell'Aquila ME,Corriero A

Published figure using OCT4 polyclonal antibody (Product # PA5-27438) in Immunohistochemistry

Thu May 14 00:00:00 EDT 2020

Upregulation of stem cell markers ALDH1A1 and OCT4 as potential biomarkers for the early detection of cervical carcinoma.

Oncology letters

Tulake W,Yuemaier R,Sheng L,Ru M,Lidifu D,Abudula A

Published figure using OCT4 polyclonal antibody (Product # PA5-27438) in Immunohistochemistry (Paraffin)

Thu Nov 01 00:00:00 EDT 2018

The TDH-GCN5L1-Fbxo15-KBP axis limits mitochondrial biogenesis in mouse embryonic stem cells.

Nature cell biology

Donato V,Bonora M,Simoneschi D,Sartini D,Kudo Y,Saraf A,Florens L,Washburn MP,Stadtfeld M,Pinton P,Pagano M

Published figure using OCT4 polyclonal antibody (Product # PA5-27438) in Western Blot

Sat Apr 01 00:00:00 EDT 2017

生物信息学

蛋白别名: 3-Oct; 4-Oct; DADB-104B20.2; gp-9; HGNC:9221; MGC22487; NF-A3; Oct-3; Oct-4; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; OTF-3; OTTHUMP00000221150; OTTHUMP00000221151; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; pou-2; POU-type homeodomain-containing DNA-binding protein; pou2

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基因别名: NF-A3; Oct-3; Oct-3/4; Oct-4; OCT3; Oct3/4; OCT4; OTF-3; Otf-4; OTF3; Otf3-rs7; Otf3g; OTF4; POU5F1

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UniProt ID: (Human) Q01860, (Mouse) P20263

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Entrez Gene ID: (Human) 5460, (Mouse) 18999

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功能