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P-Selectin Antibody (MA5-41223) in ICC/IF
Immunofluorescence analysis of SELP was performed using log phase HEL 92.1.7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 45 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with P-Selectin Recombinant Rabbit Monoclonal Antibody (JM10-95) (Product # MA5-41223) at 1:100 dilution in 0.1% BSA, incubated at ... View More
产品信息
MA5-41223
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
JM10-95
抗原
Synthetic peptide within human CD62P aa 796-830/830
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2898976
靶标信息
P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: CD62 antigen-like family member P; CD62P; FLJ45155; GMP-140; Granule membrane protein 140; granule membrane protein 140kDa; granulocyte membrane protein; LECAM3; Leukocyte-endothelial cell adhesion molecule 3; P-selectin; PADGEM; Platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein; RP1-86F14.2; selectin P (granule membrane protein 140kDa, antigen CD62)
基因别名: CD62; CD62P; GMP140; GMRP; GRMP; LECAM3; PADGEM; PSEL; SELP
UniProt ID: (Human) P16109
Entrez Gene ID: (Human) 6403
platelet degranulation
positive regulation of leukocyte migration
regulation of cellular extravasation
inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
positive regulation of platelet activation
positive regulation of phosphatidylinositol 3-kinase signaling
response to organic cyclic compound
response to lipopolysaccharide
regulation of integrin activation
calcium-mediated signaling using intracellular calcium source
positive regulation of cell adhesion
defense response to Gram-negative bacterium
leukocyte migration
leukocyte tethering or rolling
