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P-cadherin Antibody (13-5800) in Neu
The Journal of pathology 2008 -
6 Modulation of RT4 growth on collagen after 5 days. (A) RT4 growth without antibody (negative experimental control). (B) RT4 growth in the presence of anti-CK20 antibody (negative antibody control). (C) RT4 growth in the presence of anti-P-cadherin function-blocking antibody. (A-C) All show RT4 forming tight rounded multicellular colonies without any noticeable morphological changes. (D) RT4 growth in the ... View More
产品信息
13-5800
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
Human,
Mouse
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
NCC-CAD-299
抗原
Epidermoid carcinoma cell line A431.
偶联物
Unconjugated
形式
Lyophilized
浓度
2.0 mg/mL
纯化类型
Ion-exchange chromatography
保存液
PBS, pH 7.4, with 1% BSA
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2533023
产品详细信息
Source: Monoclonal antibody was obtained by fusing the mouse myeloma cell-line P3-X63-Ag8-U1 with spleen cells of BALB/c mouse after immunization with human epidermal carcinoma cell A431.1) The monoclonal antibody was harvested from ascitic fluid.
Reconstitution: Dissolve the lyophilized antibody in 50 µL of distilled water (final concentration: 2.0 mg/mL).This solution can be used as a stock solution. If dilution is necessary for your application, dilute the stock solution with the following Dilution solution just prior to use. When the entire amount of antibody is to be used over a short time period, it may be dissolved directly in 500 µL or more of the Dilution solution. Be sure to store the antibody at a minimum concentration of 2.0 mg/mL. A lower antibody concentration may result in decreased stability. Reconstituted antibody solution should contain 0.1% sodium azide as a preservative when stored at 4°C.
Specificity: This antibody specifically reacts with human P-cadherin. This antibody inhibits P-cadherin dependent cell-cell contact.
Storage: 4°C This product does not contain preservative. The stock solution (2.0 mg/mL) should be stored in aliquots at -20°C for 1 year, or should be stored at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles.
靶标信息
P-cadherin, also known as Cadherin-3, is a Type 1 cadherin protein that belongs to the cadherin superfamily. Type 1 cadherins are single-pass transmembrane proteins that have 5 extracellular cadherin repeats and an intracellular domain that binds p120-catenin and beta-catenin. Cadherins are calcium-dependent cell-cell adhesion glycoproteins responsible for a range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. P-cadherin is expressed in human fetal structures, and in adult tissues such as the basal layer of the epidermis, breast, prostate, ovary, cervix, hair follicle, and corneal endothelium. P-cadherin expression has also been reported on embryonic stem cells, and stem cells of the normal mammary gland, and hair follicle. Overexpression of P-cadherin has been associated with poor prognosis in breast, prostate, ovary, colon, and stomach carcinomas. Mutations in CDH3 are associated with congential hypotrichosis with juvenile macular dystrophy.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CADH3; cadherin 3, type 1, P-cadherin (placental); Cadherin-3; calcium-dependent adhesion protein, placental; P-cadherin; Placental cadherin; Placental-cadherin
基因别名: CDH3; CDHP; HJMD; PCAD
UniProt ID: (Human) P22223
Entrez Gene ID: (Human) 1001
retina homeostasis
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
visual perception
positive regulation of gene expression
positive regulation of keratinocyte proliferation
single organismal cell-cell adhesion
hair cycle process
keratinization
positive regulation of monophenol monooxygenase activity
negative regulation of transforming growth factor beta2 production
adherens junction organization
wound healing
response to drug
positive regulation of insulin-like growth factor receptor signaling pathway
positive regulation of melanin biosynthetic process
negative regulation of catagen
canonical Wnt signaling pathway
regulation of hair cycle by canonical Wnt signaling pathway
positive regulation of melanosome transport
