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PAK1 Antibody (71-9300) in ICC/IF
Immunofluorescence analysis of PAK-1 was done on 70% confluent log phase U87MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PAK-1 Rabbit Polyclonal Antibody (Product # 71-9300) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at ... View More
产品信息
71-9300
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human,
Mouse,
Rabbit
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide derived from an internal sequence in the human PAK1 protein. This sequence is identical in human, mouse, and rat PAK1.
偶联物
Unconjugated
形式
Liquid
浓度
0.25 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
0.1% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2534005
靶标信息
PAK1 is a serine/threonine kinase that is a target for restenosis, cancer, and immunosuppression treatment. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: activated protein kinase alpha; ADRB2; alpha pak; Alpha-PAK; CDC42/RAC effector kinase PAK-A; EC 2.7.11.1; kinase PAK1; MGC130000; MGC130001; p21 (CDKN1A)-activated kinase 1; p21 protein (Cdc42/Rac)-activated kinase 1; p21-activated kinase 1; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p65-PAK; p68-PAK; PAK 1; PAK-1; PAK1B; Protein kinase MUK2; Serine/threonine-protein kinase PAK 1; STE20 homolog, yeast
基因别名: AW045634; PAK-1; PAK1; Paka; PAKalpha
UniProt ID: (Human) Q13153, (Rat) P35465
Entrez Gene ID: (Human) 5058, (Mouse) 18479, (Rat) 29431
protein kinase activity
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
protein binding
collagen binding
ATP binding
protein kinase binding
nucleotide binding
catalytic activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Rac GTPase binding
MAP kinase kinase kinase activity
response to hypoxia
positive regulation of protein phosphorylation
protein phosphorylation
exocytosis
apoptotic process
Rho protein signal transduction
regulation of mitotic cell cycle
neuromuscular junction development
metabolic process
response to organic substance
regulation of gene expression
phosphorylation
dendrite development
cell migration
amygdala development
signal transduction by protein phosphorylation
actin cytoskeleton organization
stress-activated protein kinase signaling cascade
actin cytoskeleton reorganization
activation of protein kinase activity
cellular response to insulin stimulus
regulation of actin cytoskeleton organization
positive regulation of peptidyl-serine phosphorylation
positive regulation of intracellular estrogen receptor signaling pathway
wound healing
regulation of apoptotic process
receptor clustering
regulation of MAPK cascade
positive regulation of JUN kinase activity
protein autophosphorylation
branching morphogenesis of an epithelial tube
neuron projection morphogenesis
positive regulation of stress fiber assembly
negative regulation of cell proliferation involved in contact inhibition
dendritic spine development
gamma-aminobutyric acid secretion, neurotransmission
glutamate secretion, neurotransmission
cellular response to organic cyclic compound
observational learning
regulation of long-term synaptic potentiation
MAPK cascade
mitotic cell cycle
stimulatory C-type lectin receptor signaling pathway
axonogenesis
positive regulation of cell proliferation
positive regulation of cell migration
T cell costimulation
Fc-epsilon receptor signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
ephrin receptor signaling pathway
T cell receptor signaling pathway
activation of MAPKK activity
small GTPase mediated signal transduction
intracellular signal transduction
