Invitrogen

PAR2 Monoclonal Antibody (SAM11)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

4个已发表图片

11 篇参考文献

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

已发表图片 (4)
高级验证 (1)

PAR2 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

PAR2 Antibody (35-2300) in ICC/IF

Cell communication and signaling : CCS 2018 - Fig. 5 Expression of PAR2 in HFL-1 cells. RTqPCR was used to confirm the expression of the serine protease receptor PAR2 on HFL-1. The relative gene expression was related to the housekeeping gene 18 s ( n = 2, 1 experiment). Human lung fibroblasts, HFL-1, showed a high gene expression for PAR2, while LAD2 cells did not show any PAR2 gene expression ( a ). Expression of PAR2 examined in monolayer cultures o... View More

PAR2 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

PAR2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

PAR2 Antibody — FIG. 4. The HT29/C1 cell receptor for BFT is a membrane protein other than E-cadherin. (A) Confocal microscopic examination of control subconfluent HT29/C1 cells stained with an E-cadherin antibody (C36) demonstrates diffuse membrane staining (panel 1). HT29/C1 cells treated with Alexa 488-labeled BFT (6 nM) for 1 hour at 37degC demonstrate the near absence of E-cadherin staining (panel 2) on cells binding Alexa 488-labeled BFT (panel 4). Panel 3 demonstrates low background fluorescence in untreated control cells in the channel detecting Alexa 488-labeled BFT. (B) PAR2 does not colocalize with Alexa 488-labeled-BFT cell binding. Panel A shows the distribution of PAR2 (red) in control HT29/C1 cells. Panel B shows no colocalization of PAR2 and Alexa 488-labeled-BFT (green) binding to HT29/C1 cells (10 nM, 1 h, 37degC). Panel C shows downregulation of PAR2 immunostaining by treatment of HT29/C1 cells with trypsin (10 nM, 15 min, 37degC). Panel D shows that Alexa 488-labeled- BFT staining remains intact when PAR2 is downregulated by trypsin treatment. {TM}

产品信息

35-2300

应用

建议稀释比

已发表文章

免疫印迹 (WB)

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免疫组化 (IHC)

Assay-dependent

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免疫组化（石蜡） (IHC (P))

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免疫细胞化学 (ICC/IF)

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酶联免疫吸附实验 (ELISA)

Assay-dependent

其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

SAM11

抗原

A synthetic peptide corresponding to the N-terminal of the human PAR2 receptor.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533199

靶标信息

Coagulation factor II (thrombin) receptor-like 1 (F2RL1) is a member of the large family of 7-transmembrane-region receptors that couple to guanosine-nucleotide-binding proteins. F2RL1 is also a member of the protease-activated receptor family. It is activated by trypsin, but not by thrombin. It is activated by proteolytic cleavage of its extracellular amino terminus. The new amino terminus functions as a tethered ligand and activates the receptor. The F2RL1 gene contains two exons and is widely expressed in human tissues. The predicted protein sequence is 83% identical to the mouse receptor sequence.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Mast Cell/Proteinase Activated Receptor 2 (PAR2) Mediated Interactions in the Pathogenesis of Discogenic Back Pain.

Frontiers in cellular neuroscience

Richards J,Tang S,Gunsch G,Sul P,Wiet M,Flanigan DC,Khan SN,Moore S,Walter B,Purmessur D

35-2300 was used in Immunohistochemistry to examine the therapeutic potential of PAR2 antagonist (PAR2A) pointing toward structural restoration of the bovine IVD including down-regulation of VEGFA.

Thu Oct 01 00:00:00 EDT 2020

Mast Cell/Proteinase Activated Receptor 2 (PAR2) Mediated Interactions in the Pathogenesis of Discogenic Back Pain.

Frontiers in cellular neuroscience

Richards J,Tang S,Gunsch G,Sul P,Wiet M,Flanigan DC,Khan SN,Moore S,Walter B,Purmessur D

35-2300 was used in Immunohistochemistry to examine the therapeutic potential of PAR2 antagonist (PAR2A) pointing toward structural restoration of the bovine IVD including down-regulation of VEGFA.

Thu Oct 01 00:00:00 EDT 2020

Mast cells and mast cell tryptase enhance migration of human lung fibroblasts through protease-activated receptor 2.

Cell communication and signaling : CCS

Bagher M,Larsson-Callerfelt AK,Rosmark O,Hallgren O,Bjermer L,Westergren-Thorsson G

35-2300 was used in Immunocytochemistry-immunoflourescence to demonstrate roles for mast cells and the mast cell mediator tryptase in inducing lung fibroblast migration via PAR-2 activation.

Sat Sep 15 00:00:00 EDT 2018

Mast cells and mast cell tryptase enhance migration of human lung fibroblasts through protease-activated receptor 2.

Cell communication and signaling : CCS

Bagher M,Larsson-Callerfelt AK,Rosmark O,Hallgren O,Bjermer L,Westergren-Thorsson G

35-2300 was used in Immunocytochemistry-immunoflourescence to demonstrate roles for mast cells and the mast cell mediator tryptase in inducing lung fibroblast migration via PAR-2 activation.

Sat Sep 15 00:00:00 EDT 2018

Poly(I:C) Induces Human Lung Endothelial Barrier Dysfunction by Disrupting Tight Junction Expression of Claudin-5.

PloS one

Huang LY,Stuart C,Takeda K,D'Agnillo F,Golding B

35-2300 was used in immunocytochemistry assess the effect of polyinosinic-polycytidylic acid on the barrier function and tight junction integrity of primary human lung microvascular endothelial cells

Fri Aug 04 00:00:00 EDT 2017

Retinal neurons curb inflammation and enhance revascularization in ischemic retinopathies via proteinase-activated receptor-2.

The American journal of pathology

Sitaras N,Rivera JC,Noueihed B,Bien-Aimé M,Zaniolo K,Omri S,Hamel D,Zhu T,Hardy P,Sapieha P,Joyal JS,Chemtob S

35-2300 was used in immunohistochemistry and western blot to test if proteinase-activated receptor-2 modulates the deleterious effects of IL-1beta in ischemic retinopathies.

Sun Feb 01 00:00:00 EST 2015

Retinal neurons curb inflammation and enhance revascularization in ischemic retinopathies via proteinase-activated receptor-2.

The American journal of pathology

Sitaras N,Rivera JC,Noueihed B,Bien-Aimé M,Zaniolo K,Omri S,Hamel D,Zhu T,Hardy P,Sapieha P,Joyal JS,Chemtob S

35-2300 was used in immunohistochemistry and western blot to test if proteinase-activated receptor-2 modulates the deleterious effects of IL-1beta in ischemic retinopathies.

Sun Feb 01 00:00:00 EST 2015

Analysis of protease-activated receptor-1 and -2 in human scar formation.

The Journal of pathology

Materazzi S,Pellerito S,Di Serio C,Paglierani M,Naldini A,Ardinghi C,Carraro F,Geppetti P,Cirino G,Santucci M,Tarantini F,Massi D

35-2300 was used in immunohistochemistry (paraffin) to examine expression and biological activity of PAR-1 and PAR-2 in normal and pathological cutaneous scars.

Wed Aug 01 00:00:00 EDT 2007

Expression of protease-activated receptor-1 and -2 in orofacial granulomatosis.

Oral diseases

Ketabchi S,Massi D,Ficarra G,Rubino I,Franchi A,Paglierani M,Simoni A,Capodiferro S,Favia G,Maiorano E,Tarantini F,Cirino G,Santucci M

35-2300 was used in immunohistochemistry - paraffin section to determine the expression of PAR-1, PAR-2, MMP-2, MMP-9, COX-1, and COX-2 in patients with orofacial granulomatosis

Sun Jul 01 00:00:00 EDT 2007

The Bacteroides fragilis toxin binds to a specific intestinal epithelial cell receptor.

Infection and immunity

Wu S,Shin J,Zhang G,Cohen M,Franco A,Sears CL

35-2300 was used in immunocytochemistry to look for a cellular receptor for Bacteroides fragilis toxin on intestinal epithelial cells

Fri Sep 01 00:00:00 EDT 2006

Evidence that protease activated receptor 2 expression is enhanced in human coronary atherosclerotic lesions.

Journal of clinical pathology

Napoli C,de Nigris F,Wallace JL,Hollenberg MD,Tajana G,De Rosa G,Sica V,Cirino G

Sat May 01 00:00:00 EDT 2004

Constitutive protease-activated receptor-2-mediated migration of MDA MB-231 breast cancer cells requires both beta-arrestin-1 and -2.

The Journal of biological chemistry

Ge L,Shenoy SK,Lefkowitz RJ,DeFea K

Fri Dec 31 00:00:00 EST 2004

生物信息学

蛋白别名: coagulation factor II (thrombin) receptor-like 1; Coagulation factor II receptor-like 1; G-protein coupled receptor 11; PAR-2; protease-activated receptor 2; Proteinase-activated receptor 2; Thrombin receptor-like 1

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基因别名: F2RL1; GPR11; PAR2

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UniProt ID: (Human) P55085

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Entrez Gene ID: (Human) 2150

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功能

G-protein alpha-subunit binding receptor activity G-protein coupled receptor activity receptor binding protein binding thrombin receptor activity G-protein beta-subunit binding G-protein coupled receptor

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