Invitrogen

PAR4 Polyclonal Antibody

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PAR4 Antibody (PA1-755) in WB

Western blot of Par-4 on rat spleen extract using Product # PA1-755.

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产品信息

PA1-755

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

其他PubMed (Misc)

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产品规格

种属反应

Human, Rat

已发表种属

Not Applicable

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic Peptide: C E(322) Q L K Q E N K T L L K V V G Q L T R(340)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2159105

产品详细信息

PA1-755 detects prostate apoptosis response protein (Par-4) from human and rat tissues.

PA1-755 has been sucessfully used in Western blot procedures. By Western blot, this antibody detects an ~40 kDa protein representing Par-4 in AT-3 cell extract. In Western blots of rat tissue extracts, including testes, spleen, ovary, lung, and skeletal muscle, ~40 kDa and ~38 kDa proteins representing Par-4 are detected. In Western blots of T47D cell extracts, an ~40 kDa protein representing Par-4 and a smaller ~20 kDa unknown protein is detected.

PA1-755 immunizing peptide corresponds to amino acids residues 322-340 from human Par-4. PA1-755 immunizing peptide (Cat. # PEP-060) is available for use in neutralization and control experiments.

靶标信息

This gene encodes a tumor suppressor protein that selectively induces apoptosis in cancer cells through intracellular and extracellular mechanisms. The intracellular mechanism involves the inhibition of pro-survival pathways and the activation of Fas-mediated apoptosis, while the extracellular mechanism involves the binding of a secreted form of this protein to glucose regulated protein 78 (GRP78) on the cell surface, which leads to activation of the extrinsic apoptotic pathway. This gene is located on the unstable human chromosomal 12q21 region and is often deleted or mutated different tumors. The encoded protein also plays an important role in the progression of age-related diseases.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Par-4 is a mediator of neuronal degeneration associated with the pathogenesis of Alzheimer disease.

Nature medicine

Guo Q,Fu W,Xie J,Luo H,Sells SF,Geddes JW,Bondada V,Rangnekar VM,Mattson MP

PA1-755 was used in immunohistochemistry, western blot and immunocytochemistry to investigate the role of Par-4 in neuronal degeneration with respect to Alzheimer disease

Sat Aug 01 00:00:00 EDT 1998

A novel repressor, par-4, modulates transcription and growth suppression functions of the Wilms' tumor suppressor WT1.

Molecular and cellular biology

Johnstone RW,See RH,Sells SF,Wang J,Muthukkumar S,Englert C,Haber DA,Licht JD,Sugrue SP,Roberts T,Rangnekar VM,Shi Y

PA1-755 was used in immunoprecipitation and western blot to investigate the effect of par-4 on WT1 transcription

Sun Dec 01 00:00:00 EST 1996

生物信息学

蛋白别名: Par-4; par-4 induced by effectors of apoptosis; PRKC apoptosis WT1 regulator protein; PRKC, apoptosis, WT1, regulator; prostate apoptosis response 4; Prostate apoptosis response 4 protein; Prostate apoptosis response protein 4; prostate apoptosis response protein PAR-4; prostate apoptosis response-4; Transcriptional repressor Par-4-like protein PAWR; transcriptional repressor PAR4; WT1-interacting protein

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基因别名: Par-4; PAR4; PAWR

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UniProt ID: (Human) Q96IZ0, (Rat) Q62627

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Entrez Gene ID: (Human) 5074, (Rat) 64513

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功能

transcription corepressor activity actin binding protein kinase C binding protein binding protein phosphatase 1 binding enzyme binding leucine zipper domain binding

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