Invitrogen

PAX7 Polyclonal Antibody

PAX7 Antibody in Immunocytochemistry (ICC/IF)

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PAX7 Antibody (PA1-117) in ICC/IF

Immunofluorescent analysis of PAX7 (green) in MCF7 and HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a PAX7 polyclonal antibody (Product # PA1-117) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for... View More

PAX7 Antibody in Immunoprecipitation (IP)

PAX7 Antibody in Immunohistochemistry (IHC)

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产品信息

PA1-117

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

1:20-1:200

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流式细胞分析 (Flow)

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免疫沉淀 (IP)

3 µg

其他PubMed (Misc)

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产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Purified internal fragment of human recombinant PAX7 expressed in E. coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS with 30% glycerol, 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2539886

产品详细信息

Western blot analysis of PA1-117 detects a prominent ~54 kDa protein in human HeLa, MCF7, rhabdomyosarcoma and skeletal muscle satellite cells and a weaker ~57 kDa protein in HeLa, rhabdomyosarcoma and skeletal muscle satellite cells. The ~57 kDa band seems to be enriched in immunoprecipitations from HeLa lysate, suggesting a bias for the larger isoform in ths application. Weak unknown bands of lower molecular weight and at ~110-130 kDa are also detected. Specificity of the antibody was also confirmed in Hela cells overexpressing full length PAX7.

靶标信息

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Exosome biopotentiated hydrogel restores damaged skeletal muscle in a porcine model of stress urinary incontinence.

NPJ Regenerative medicine

Rolland TJ,Peterson TE,Singh RD,Rizzo SA,Boroumand S,Shi A,Witt TA,Nagel M,Kisby CK,Park S,Rowe LA,Paradise CR,Becher LRE,Paradise BD,Stalboerger PG,Trabuco EC,Behfar A

PA1-117 was used in Western Blotting to describe PEP-based biopotentiation of hydrogels implemented to restore skeletal muscle function and may serve as a promising approach for the nonsurgical management of SUI.

Thu Sep 29 00:00:00 EDT 2022

Exosome biopotentiated hydrogel restores damaged skeletal muscle in a porcine model of stress urinary incontinence.

NPJ Regenerative medicine

Rolland TJ,Peterson TE,Singh RD,Rizzo SA,Boroumand S,Shi A,Witt TA,Nagel M,Kisby CK,Park S,Rowe LA,Paradise CR,Becher LRE,Paradise BD,Stalboerger PG,Trabuco EC,Behfar A

Thu Sep 29 00:00:00 EDT 2022

G-quadruplex DNA structures in human stem cells and differentiation.

Nature communications

Zyner KG,Simeone A,Flynn SM,Doyle C,Marsico G,Adhikari S,Portella G,Tannahill D,Balasubramanian S

PA1-117 was used in Immunocytochemistry to determine whether G-quadruplexes (G4s) are key genomic structural features linked to cellular differentiation.

Mon Jan 10 00:00:00 EST 2022

Uhrf1 governs the proliferation and differentiation of muscle satellite cells.

iScience

Sakai H,Sawada Y,Tokunaga N,Tanaka K,Nakagawa S,Sakakibara I,Ono Y,Fukada SI,Ohkawa Y,Kikugawa T,Saika T,Imai Y

PA1-117 was used in Immunohistochemistry to show that Uhrf1 is a critical epigenetic regulator of proliferation and differentiation in satellite cells, by controlling cell-type-specific gene expression via maintenance of DNA methylation.

Fri Mar 18 00:00:00 EDT 2022

Muscle injury causes long-term changes in stem-cell DNA methylation.

Proceedings of the National Academy of Sciences of the United States of America

Falick Michaeli T,Sabag O,Fok R,Azria B,Monin J,Nevo Y,Gielchinsky Y,Berman BP,Cedar H,Bergman Y

PA1-117 was used in Flow Cytometry to demonstrate that this activation is accompanied by a dramatic change in the stem-cell methylation pattern that prepares them epigenetically for terminal myocyte differentiation.

Tue Dec 27 00:00:00 EST 2022

Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacity.

Genes to cells : devoted to molecular & cellular mechanisms

Hara A,Kato K,Ishihara T,Kobayashi H,Asai N,Mii S,Shiraki Y,Miyai Y,Ando R,Mizutani Y,Iida T,Takefuji M,Murohara T,Takahashi M,Enomoto A

PA1-117 was used in Immunohistochemistry to investigate if Meflin is a useful potential marker to localize Mesenchymal stem cells and/or their immature progenitors in multiple tissues.

Thu Jul 01 00:00:00 EDT 2021

Dysregulation of Muscle-Specific MicroRNAs as Common Pathogenic Feature Associated with Muscle Atrophy in ALS, SMA and SBMA: Evidence from Animal Models and Human Patients.

International journal of molecular sciences

Malacarne C,Galbiati M,Giagnorio E,Cavalcante P,Salerno F,Andreetta F,Cagnoli C,Taiana M,Nizzardo M,Corti S,Pensato V,Venerando A,Gellera C,Fenu S,Pareyson D,Masson R,Maggi L,Dalla Bella E,Lauria G,Mantegazza R,Bernasconi P,Poletti A,Bonanno S,Marcuzzo S

Published figure using PAX7 polyclonal antibody (Product # PA1-117) in Western Blot

Wed May 26 00:00:00 EDT 2021

Interference with SRF expression in skeletal muscles reduces peripheral nerve regeneration in mice.

Scientific reports

Wanner R,Knöll B

PA1-117 was used in Immunohistochemistry to conditionally ablated SRF (serum response factor), an important myofiber transcription factor, in skeletal muscles of mice.

Tue Mar 24 00:00:00 EDT 2020

Interference with SRF expression in skeletal muscles reduces peripheral nerve regeneration in mice.

Scientific reports

Wanner R,Knöll B

PA1-117 was used in Immunohistochemistry to conditionally ablated SRF (serum response factor), an important myofiber transcription factor, in skeletal muscles of mice.

Tue Mar 24 00:00:00 EDT 2020

Age-dependent increase in angiopoietin-like protein 2 accelerates skeletal muscle loss in mice.

The Journal of biological chemistry

Zhao J,Tian Z,Kadomatsu T,Xie P,Miyata K,Sugizaki T,Endo M,Zhu S,Fan H,Horiguchi H,Morinaga J,Terada K,Yoshizawa T,Yamagata K,Oike Y

PA1-117 was used in Immunohistochemistry-immunofluorescence to aNGPTL2 up-regulation in skeletal myocytes accelerates muscle atrophy, and exercise-induced attenuation of ANGPTL2 expression in those tissues may partially explain how exercise training prevents sarcopenia.

Fri Feb 02 00:00:00 EST 2018

Age-dependent increase in angiopoietin-like protein 2 accelerates skeletal muscle loss in mice.

The Journal of biological chemistry

Zhao J,Tian Z,Kadomatsu T,Xie P,Miyata K,Sugizaki T,Endo M,Zhu S,Fan H,Horiguchi H,Morinaga J,Terada K,Yoshizawa T,Yamagata K,Oike Y

Fri Feb 02 00:00:00 EST 2018

Histone H3.3 sub-variant H3mm7 is required for normal skeletal muscle regeneration.

Nature communications

Harada A,Maehara K,Ono Y,Taguchi H,Yoshioka K,Kitajima Y,Xie Y,Sato Y,Iwasaki T,Nogami J,Okada S,Komatsu T,Semba Y,Takemoto T,Kimura H,Kurumizaka H,Ohkawa Y

PA1-117 was used in Immunohistochemistry to characterise the function of H3mm7, a histone H3.3 sub-variant expressed in skeletal muscle cells.

Wed Apr 11 00:00:00 EDT 2018

Efficient differentiation of human pluripotent stem cells into skeletal muscle cells by combining RNA-based MYOD1-expression and POU5F1-silencing.

Scientific reports

Akiyama T,Sato S,Chikazawa-Nohtomi N,Soma A,Kimura H,Wakabayashi S,Ko SBH,Ko MSH

PA1-117 was used in Western Blotting to show a rapid and robust method to induce myogenic differentiation of human pluripotent stem cells by introducing mRNA encoding MYOD1 together with siRNA-mediated knockdown of POU5F1.

Fri Jan 19 00:00:00 EST 2018

Targeting β1-integrin signaling enhances regeneration in aged and dystrophic muscle in mice.

Nature medicine

Rozo M,Li L,Fan CM

PA1117 was used in immunocytochemistry and immunohistochemistry - frozen section to explore how the interaction between beta1-integrin and Fgf2 contributes to the satellite cell niche

Mon Aug 01 00:00:00 EDT 2016

Enobosarm (GTx-024) Modulates Adult Skeletal Muscle Mass Independently of the Androgen Receptor in the Satellite Cell Lineage.

Endocrinology

Dubois V,Simitsidellis I,Laurent MR,Jardi F,Saunders PT,Vanderschueren D,Claessens F

PA1-117 was used in immunohistochemistry - paraffin section to study the role of muscle androgen receptor in GTx-024's anabolic effect

Tue Dec 01 00:00:00 EST 2015

生物信息学

蛋白别名: FLJ37460; HuP1; paired box gene 7; paired box homeotic gene 7; Paired box protein Pax-7; paired box transcription factor PAX7; paired domain gene 7; PAX7 transcriptional factor; PAX7A

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基因别名: HUP1; Pax-7; PAX7; PAX7B; RMS2

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UniProt ID: (Human) P23759, (Mouse) P47239

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Entrez Gene ID: (Human) 5081, (Mouse) 18509

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功能

transcription factor activity, RNA polymerase II core promoter sequence-specific transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding DNA binding protein binding

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