Invitrogen

PAX8 Polyclonal Antibody

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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抗体检测数据 (3)
高级验证 (2)

PAX8 Antibody in Immunocytochemistry (ICC/IF)

PAX8 Antibody in Immunocytochemistry (ICC/IF)

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PAX8 Antibody (PA5-18457) in ICC/IF

Immunofluorescence analysis of PAX8 was performed using 70% confluent log phase SK-OV-3 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with PAX8 Polyclonal Antibody (Product # PA5-18457) at 1 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11078, 1:2000 dilution... View More

PAX8 Antibody in Immunocytochemistry (ICC/IF)

PAX8 Antibody in Western Blot (WB)

PAX8 Antibody in Western Blot (WB)

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PAX8 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PAX8 was performed using 70% confluent log phase SK-OV-3 cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with PAX8 Polyclonal Antibody (Product # PA5-18457) at 1 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11078, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents IMR-32 cells having no expression of PAX8. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

PAX8 Antibody in Western Blot (WB) — Western blot was performed using Anti-PAX8 Polyclonal Antibody (Product # PA5-18457) and 38 and 50 kDa bands corresponding to PAX8 was observed across the cell lines tested except IMR-32 and Jurkat. Modified whole cell extracts (1% SDS) (30 µg lysate) of SK-OV-3 (Lane 1), OVCAR-3 (Lane 2), MCF7 (Lane 3), PC-3 (Lane 4), HEK-293 (Lane 5), HeLa (Lane 6), IMR-32 (Lane 7) and Jurkat (Lane 8) were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Rabbit anti-Goat IgG Heavy Chain, Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

PAX8 Antibody in Western Blot (WB) — Western blot analysis of PAX8 using PAX8 Polyclonal Antibody (Product # PA5-18457) in HEK293 overexpressing PAX8A (mock transfection in first lane).

PAX8 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. The expression was observed in SK-BR-3, OVCAR-3, MCF7, PC-3, HEK-293 and HeLa and not in IMR-32 and Jurkat using PAX8 Polyclonal Antibody (Product # PA5-18457) in Western Blot. {RE}

PAX8 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-PAX8 Polyclonal Antibody (Product # PA5-18457) shows increased expression of Musashi-1 in SKOV-3 when compared to IMR-32. {RE}

产品信息

PA5-18457

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-1.5 µg/mL

-

免疫细胞化学 (ICC/IF)

1 µg/mL

-

产品规格

种属反应

Human

宿主/亚型

Goat / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide sequence (AQPGSDKRKMDD) corresponding to the internal amino acids of PAX8 (aa 189-201).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

TBS, pH 7.3, with 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_10978947

产品详细信息

This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.

靶标信息

This gene encodes a member of the paired box family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: HGNC:8622; Paired box protein Pax-8; paired domain gene 8

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基因别名: PAX8

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UniProt ID: (Human) Q06710

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Entrez Gene ID: (Human) 7849

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RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding thyroid-stimulating hormone receptor activity protein binding transcription regulatory region DNA binding

参与通路

urogenital system development branching involved in ureteric bud morphogenesis kidney development mesonephros development mesenchymal to epithelial transition involved in metanephros morphogenesis transcription, DNA-templated transcription from RNA polymerase II promoter sulfur compound metabolic process central nervous system development anatomical structure morphogenesis thyroid gland development thyroid-stimulating hormone signaling pathway pronephric field specification inner ear morphogenesis regulation of apoptotic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter pronephros development cellular response to gonadotropin stimulus otic vesicle development positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis metanephric epithelium development metanephric distal convoluted tubule development metanephric comma-shaped body morphogenesis metanephric S-shaped body morphogenesis metanephric nephron tubule formation negative regulation of mesenchymal cell apoptotic process involved in metanephric nephron morphogenesis regulation of metanephric nephron tubule epithelial cell differentiation positive regulation of branching involved in ureteric bud morphogenesis negative regulation of mesenchymal cell apoptotic process involved in metanephros development negative regulation of apoptotic process involved in metanephric collecting duct development negative regulation of apoptotic process involved in metanephric nephron tubule development positive regulation of metanephric DCT cell differentiation positive regulation of thyroid hormone generation regulation of thyroid-stimulating hormone secretion