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PCBP2 Antibody (PA5-98446) in IHC (P)
Immunohistochemical analysis of PCBP2 in paraffin embedded human testis tissue using a PCBP2 polyclonal antibody (Product # PA5-98446) at a dilution of 1:200. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detec... View More
产品信息
PA5-98446
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant Human Poly(rC)-binding protein 2 protein (171-293AA)
偶联物
形式
Liquid
浓度
2.69 mg/mL
纯化类型
Protein G
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.03% ProClin 300
保存条件
-20°C or -80°C if preferred
运输条件
Wet ice
RRID
AB_2813059
靶标信息
The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Alpha CP2; Alpha-CP2; Heterogeneous nuclear ribonucleoprotein E2; heterogenous nuclear ribonucleoprotein E2; hnRNP E2; MGC110998; Poly(rC)-binding protein 2; rCbinding protein 2
基因别名: hnRNP-E2; HNRNPE2; HNRPE2; PCBP2
UniProt ID: (Human) Q15366
Entrez Gene ID: (Human) 5094
mRNA splicing, via spliceosome
gene expression
mRNA metabolic process
negative regulation of type I interferon production
viral RNA genome replication
proteasome-mediated ubiquitin-dependent protein catabolic process
innate immune response
negative regulation of defense response to virus
defense response to virus
IRES-dependent viral translational initiation
