Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
产品信息
PA5-120718
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 490-622 of human PCK1 (NP_002582.3).
偶联物
形式
Liquid
浓度
2.53 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2914290
产品详细信息
Positive test controls include: HepG2, Mouse liver, Mouse kidney, Rat liver, Rat pancreas, Rat kidney. The target is usually found in the following locations: Cytoplasm.
Immunogen sequence: FGKYLAHWLS MAQHPAAKLP KIFHVNWFRK DKEGKFLWPG FGENSRVLEW MFNRIDGKAS TKLTPIGYIP KEDALNLKGL GHINMMELFS ISKEFWEKEV EDIEKYLEDQ VNADLPCEIE REILALKQRI SQM
靶标信息
Phosphoenolpyruvate Carboxykinase 1 (PCK1) is a main control point for the regulation of gluconeogenesis. During gluconeogenesis PCK1 acts as the rate-limiting enzyme. PCK1 regulates the formation and maintenance of memory CH8(+) T-cells via gluconeogenesis. PCK1 aids in controlling the levels of metabolic intermediates in the citric acid cycle by regulating cataplerosis and anaplerosis. When glucose levels are high, PCK1 catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP). When they are low it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate. When phosphorylated at Ser-90 by AKT1, PCK1 acts as a protein kinase by reducing the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as the donor. This activity regulates lipogenesis by disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins and transcription of lipogenesis-related genes. An important paralog of this gene is PCK2.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
篇参考文献 (0)
您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
生物信息学
蛋白别名: HGNC:8724; MGC22652; PE PEPCKC; PEP carboxykinase; PEPCK-C; phosphoenolpyruvate carboxykinase (GTP); phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase 1, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic; phosphoenolpyruvate carboxykinase, cytosolic (GTP); Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase; Serine-protein kinase PCK1
基因别名: AI265463; GTP; PCK; Pck-1; PCK1; PEPCK; PEPCK-C; PEPCK1; PEPCKC; RATPEPCK
UniProt ID: (Human) P35558, (Rat) P07379, (Mouse) Q9Z2V4
Entrez Gene ID: (Human) 5105, (Rat) 362282, (Mouse) 18534
glucose metabolic process
gluconeogenesis
oxaloacetate metabolic process
internal protein amino acid acetylation
response to activity
response to insulin
glucose homeostasis
glycerol biosynthetic process from pyruvate
cellular response to potassium ion starvation
positive regulation of transcription from RNA polymerase II promoter in response to acidic pH
lipid metabolic process
