Invitrogen

PCNA Monoclonal Antibody (PC10), Alexa Fluor™ Plus 555

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他74个PCNA抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (3)
高级验证 (1)

PCNA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PCNA Antibody (754-9910-82) in IHC (P)

Immunohistochemical analysis of PCNA was performed on formalin-fixed paraffin-embedded human duodenum tissue. To expose the target protein, HIER was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted in 1X solution in water in a pressure cooker for 10 minutes, followed by a 5-min cool down and a 5-min wash with ddH2O. Tissues... View More

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PCNA Antibody — Clone PC10 specificity was demonstrated by siRNA mediated knockdown of the target protein. Reduced PCNA signal was observed in HeLa cells transfected with PCNA siRNA by Western Blot. {KD}

产品信息

754-9910-82

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

20 µg/mL

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

PC10

抗原

Rat recombinant PCNA polypeptides.

偶联物

Alexa Fluor™ Plus 555 Alexa Fluor™ Plus 555 Alexa Fluor™ Plus 555

激发/发射光谱

558/572 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

purified

保存液

PBS, pH 7.2, with 0.5% BSA, 10% proprietary stabilizer

内含物

0.05% sodium azide

保存条件

4° C, store in dark

运输条件

Wet ice

产品详细信息

Description: The PC10 antibody reacts with human PCNA which is involved in the control of eukaryotic DNA replication and found in the nucleus.

Applications Reported: This PC10 antibody has been reported for use in flow cytometric analysis, immunocytochemistry, western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This PC10 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded tissue using high pH antigen retrieval and can be used at 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

靶标信息

PCNA (polymerase delta auxiliary protein) is essential for DNA replication and is involved in DNA excision and mismatch repair pathways. PCNA binds to the CDK inhibitor p21, the structure-specific endonucleases Fen1 and XPG, and DNA cytosine 5-methyltransferase (MCMT). PCNA is a potentially useful marker of cells with proliferative potential and for identifying the proliferation status of tumor tissue (i.e. relevant to prognosis). PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. PCNA is found in the nucleus and is a cofactor of DNA polymerase delta, and acts as a homotrimer and helps increase the processing of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for PCNA. Pseudogenes of PCNA have been described on chromosome 4 and on the X chromosome.

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