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PDE1C Antibody (PA5-115728) in ICC/IF
Immunocytochemistry analysis of PDE1C in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115728) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
产品信息
PA5-115728
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human PDE1C(Accession Q14123), corresponding to amino acid residues G559-D609.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2900363
产品详细信息
Antibody detects endogenous levels of total PDE1C.
靶标信息
Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1C, are calmodulin -dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 ).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C; Cam-PDE 1C; cyclic nucleotide phosphodiesterase 1 C; Dual specificity calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C; Hcam3; Human 3',5' cyclic nucleotide phosphodiesterase (HSPDE1C1A); phosphodiesterase 1C calmodulin-dependent (70kD); phosphodiesterase 1C, calmodulin-dependent 70kDa
基因别名: cam-PDE 1C; hCam-3; Hcam3; PDE1C; Pde1c2
UniProt ID: (Human) Q14123, (Mouse) Q64338, (Rat) Q63421
Entrez Gene ID: (Human) 5137, (Mouse) 18575, (Rat) 81742
calmodulin-dependent cyclic-nucleotide phosphodiesterase activity
calmodulin binding
metal ion binding
3',5'-cyclic-nucleotide phosphodiesterase activity
3',5'-cyclic-AMP phosphodiesterase activity
phosphoric diester hydrolase activity
hydrolase activity
cAMP binding
calcium- and calmodulin-regulated 3',5'-cyclic-GMP phosphodiesterase activity
phosphodiesterase
