Invitrogen

PDGF-AA Polyclonal Antibody, Biotin, PeproTech®

图: 1 / 3

PDGF-AA Antibody (500-P46BT-25UG) in WB

Western Blot: To detect hPDGF-AA by Western Blot analysis PDGF-AA Polyclonal Antibody, Biotin (Product # 500-P46BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPDGF-AA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Product Image Gallery

产品信息

500-P46BT-25UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.25-1.0 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived, 28.5 kDa Recombinant Human PDGF-AA

查看免疫原

偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2930561

产品详细信息

AA Sequence of recombinant protein: SIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human PDGF-AA. Anti-Human PDGF-AA-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA: To detect hPDGF-AA by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human PDGF-AA (500-P46) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hPDGF-AA.

Western Blot: To detect hPDGF-AA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hPDGF-AA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

500-P46BT-1MG will be provided as 2 x 500 µg

靶标信息

Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the alpha -granules of platelets activated during the coagulation of blood to form serum. Subsequent studies have demonstrated that PDGF is not one molecule but three, each a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed alpha and beta. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-gamma production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: H-PDGF-AA; PDGF A-chain; PDGF subunit A; PDGF-1; Platelet-derived growth factor A chain; platelet-derived growth factor A-chain; platelet-derived growth factor alpha chain; Platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor subunit A

查看更多收起

基因别名: PDGF-A; PDGF1; PDGFA

查看更多收起

UniProt ID: (Human) P04085

查看更多收起

Entrez Gene ID: (Human) 5154

查看更多收起

功能

Ras guanyl-nucleotide exchange factor activity platelet-derived growth factor receptor binding protein binding collagen binding growth factor activity protein homodimerization activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity platelet-derived growth factor binding growth factor

参与通路