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PIASy Antibody (PA5-99455) in IHC (P)
Immunohistochemistry analysis of PIASy in mouse colorectal tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with PIASy polyclonal antibody (Product # PA5-99455) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
产品信息
PA5-99455
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human PIAS4(Accession Q8N2W9), corresponding to amino acid residues L468-C510.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2818388
产品详细信息
Antibody detects endogenous levels of total PIAS4.
靶标信息
Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53/TP53 pathway, the Wnt pathway and the steroid hormone signaling pathway. Involved in gene silencing. Mediates sumoylation of CEBPA, PARK7, HERC2, MYB, TCF4 and RNF168. In Wnt signaling, represses LEF1 and enhances TCF4 transcriptional activities through promoting their sumoylations. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: e3 sumo- ligase pias4; E3 SUMO-protein ligase PIAS4; FLJ12419; MGC35296; PIAS-gamma; PIASy; protein inhibitor of activated STAT PIASy; Protein inhibitor of activated STAT protein 4; Protein inhibitor of activated STAT protein gamma; protein inhibitor of activated STAT protein PIASy; protein inhibitor of activated STAT, 4; zinc finger, MIZ-type containing 6
基因别名: PIAS-gamma; PIAS4; PIASG; PIASY; ZMIZ6
UniProt ID: (Human) Q8N2W9, (Mouse) Q9JM05
Entrez Gene ID: (Human) 51588, (Rat) 362827, (Mouse) 59004
DNA binding
transcription corepressor activity
protein binding
protein C-terminus binding
zinc ion binding
ligase activity
SUMO transferase activity
ubiquitin protein ligase binding
SUMO ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
negative regulation of transcription from RNA polymerase II promoter
biological_process
metabolic process
protein sumoylation
negative regulation of NF-kappaB transcription factor activity
positive regulation of protein sumoylation
negative regulation of transcription, DNA-templated
positive regulation of keratinocyte apoptotic process
positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage
transcription, DNA-templated
regulation of transcription, DNA-templated
JAK-STAT cascade
multicellular organism development
negative regulation of tumor necrosis factor-mediated signaling pathway
viral process
Wnt signaling pathway
negative regulation of nucleic acid-templated transcription
double-strand break repair via nonhomologous end joining
