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产品信息
PA5-37136
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthetic peptide corresponding to residues in human PIF1
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.2, with 50% glycerol
内含物
0.02% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2553925
产品详细信息
This antibody detects endogenous protein at a molecular weight of 70 kDa.
Purity is >95% by SDS-PAGE.
靶标信息
PIF1 is a single-stranded (ss) DNA-dependent ATPase as well as a DNA helicase that unwinds DNA in a 5' to 3' direction with respect to the DNA strand on which it binds first. This protein is critical to DNA replication and telomere length maintenance in Saccharomyces cerevisiae. The PIF1 gene is highly conserved from yeast to humans. Mutations in the gene that encodes for PIF1 cause all telomeres to lengthen, suggesting that the PIF1 functions as a catalytic inhibitor of both new telomere formation and telomere elongation. Human PIF1 preferentially binds telomeric DNA where it inhibits telomerase activity. PIF1 specifically counteracts the RecQ homolog Sgs1 helicase activity, and degradation of PIF1 is mediated by the ubiquitin-26S proteasome pathway.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ATP-dependent DNA helicase PIF1; ATP-dependent DNA helicase PIF1 {ECO:0000255|HAMAP-Rule:MF_03176}; DNA helicase-like protein; DNA repair and recombination helicase PIF1; DNA repair and recombination helicase PIF1 {ECO:0000255|HAMAP-Rule:MF_03176}; petite integration frequency 1; PIF1 5'-to-3' DNA helicase homolog; PIF1 homolog; PIF1/RRM3 DNA helicase-like protein; Pif1/Rrm3 DNA-helicase-like protein
基因别名: 4631410M14; AI449441; C15orf20; PIF; PIF1
UniProt ID: (Human) Q9H611, (Rat) Q1HG60, (Mouse) Q80SX8
Entrez Gene ID: (Human) 80119, (Rat) 367645, (Mouse) 208084
magnesium ion binding
ATP binding
telomerase inhibitor activity
single-stranded DNA-dependent ATP-dependent DNA helicase activity
ATP-dependent 5'-3' DNA/RNA helicase activity
telomeric DNA binding
ATP-dependent 5'-3' DNA helicase activity
G-quadruplex DNA binding
nucleotide binding
DNA binding
DNA helicase activity
helicase activity
hydrolase activity
DNA helicase
mitochondrial genome maintenance
telomere maintenance
DNA repair
DNA recombination
regulation of telomere maintenance
negative regulation of telomere maintenance via telomerase
DNA duplex unwinding
G-quadruplex DNA unwinding
negative regulation of telomerase activity
cellular response to DNA damage stimulus
