Invitrogen

PIM1 Monoclonal Antibody (ZP003)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

6个已发表图片

6 篇参考文献

查看其他29个PIM1抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

图: 1 / 10

PIM1 Antibody (39-4600) in WB

Western blot analysis of K562 cell lysates using Zymed Ms anti-Pim-1 (Product # 39-4600).

PIM1 Antibody in Western Blot, Immunoprecipitation (WB, IP)

Product Image Gallery

PIM1 Antibody — The specificity of anti-PIM1 monoclonal antibody (Product # 39-4600) was demonstrated using chromatin immunoprecipitation (ChIP). q-PCR was used to demonstrate levels of enrichment of PIM1 at the expected loci in exon 1 of human EGR1 gene but not negative site in -15kb region of the same gene in Human J9.2 J-lat T lymphocytes culture treated with 10 µg/mL PHA (phytohemaglutin) for 2 hours compared to those in untreated cells. {TM}

产品信息

39-4600

应用

建议稀释比

已发表文章

免疫印迹 (WB)

Assay-dependent

查看5篇已发表文章 5篇已发表文章

酶联免疫吸附实验 (ELISA)

Assay-dependent

免疫沉淀 (IP)

查看1篇已发表文章 1篇已发表文章

染色质免疫沉淀实验 (ChIP)

1:100

产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

ZP003

抗原

Synthetic peptide derived from the internal region of the human Pim-1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2533414

产品详细信息

42-4800 has been successfully used in ChIP analysis of PIM1 in human J9.2 J-lat T lymphocytes.

靶标信息

The proto-oncogene PIM1 is upregulated in prostate cancer. PIM1, which belongs to the Serine/Threonine protein kinase family, is thought to play a role in signal transduction in blood cells. The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer. It may affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. PIM1 is expressed primarily in cells of the hematopoietic and germ line lineages.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Pim1 maintains telomere length in mouse cardiomyocytes by inhibiting TGFβ signalling.

Cardiovascular research

Ebeid DE,Khalafalla FG,Broughton KM,Monsanto MM,Esquer CY,Sacchi V,Hariharan N,Korski KI,Moshref M,Emathinger J,Cottage CT,Quijada PJ,Nguyen JH,Alvarez R,Völkers M,Konstandin MH,Wang BJ,Firouzi F,Navarrete JM,Gude NA,Goumans MJ,Sussman MA

39-4600 was used in Western Blotting to delineate the mechanism of telomere length regulation by the interplay between Pim1 and components of TGFβ signaling pathways.

Fri Jan 01 00:00:00 EST 2021

PIM1 Promotes Survival of Cardiomyocytes by Upregulating c-Kit Protein Expression.

Cells

Ebeid DE,Firouzi F,Esquer CY,Navarrete JM,Wang BJ,Gude NA,Sussman MA

39-4600 was used in Western Blot, Immunoprecipitation to identify the mechanistic relationship between PIM1 and c-Kit in cardiomyocytes, demonstrating another facet of cardioprotection regulated by PIM1 kinase.

Mon Aug 31 00:00:00 EDT 2020

PIM1 Promotes Survival of Cardiomyocytes by Upregulating c-Kit Protein Expression.

Cells

Ebeid DE,Firouzi F,Esquer CY,Navarrete JM,Wang BJ,Gude NA,Sussman MA

Mon Aug 31 00:00:00 EDT 2020

PIM1 Promotes Survival of Cardiomyocytes by Upregulating c-Kit Protein Expression.

Cells

Ebeid DE,Firouzi F,Esquer CY,Navarrete JM,Wang BJ,Gude NA,Sussman MA

Mon Aug 31 00:00:00 EDT 2020

PIM1 Promotes Survival of Cardiomyocytes by Upregulating c-Kit Protein Expression.

Cells

Ebeid DE,Firouzi F,Esquer CY,Navarrete JM,Wang BJ,Gude NA,Sussman MA

Mon Aug 31 00:00:00 EDT 2020

Nucleostemin rejuvenates cardiac progenitor cells and antagonizes myocardial aging.

Journal of the American College of Cardiology

Hariharan N,Quijada P,Mohsin S,Joyo A,Samse K,Monsanto M,De La Torre A,Avitabile D,Ormachea L,McGregor MJ,Tsai EJ,Sussman MA

Published figure using PIM1 monoclonal antibody (Product # 39-4600) in Western Blot

Tue Jan 20 00:00:00 EST 2015

Functional Effect of Pim1 Depends upon Intracellular Localization in Human Cardiac Progenitor Cells.

The Journal of biological chemistry

Samse K,Emathinger J,Hariharan N,Quijada P,Ilves K,Völkers M,Ormachea L,De La Torre A,Orogo AM,Alvarez R,Din S,Mohsin S,Monsanto M,Fischer KM,Dembitsky WP,Gustafsson ÅB,Sussman MA

39-4600 was used in western blot to show that subcellular targeting bolsters the cardioprotective effects of Pim1 in primary human cardiac progenitor cells

Fri May 29 00:00:00 EDT 2015

Fibronectin is essential for reparative cardiac progenitor cell response after myocardial infarction.

Circulation research

Konstandin MH,Toko H,Gastelum GM,Quijada P,De La Torre A,Quintana M,Collins B,Din S,Avitabile D,Völkers M,Gude N,Fässler R,Sussman MA

Published figure using PIM1 monoclonal antibody (Product # 39-4600) in Western Blot

Fri Jul 05 00:00:00 EDT 2013

生物信息学

蛋白别名: Oncogene PIM1; pim-1 kinase 44 kDa isoform; pim-1 oncogene (proviral integration site 1); proto-oncogene serine/threonine-protein kinase pim-1; Serine/threonine-protein kinase pim-1

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基因别名: PIM; PIM1

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UniProt ID: (Human) P11309

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Entrez Gene ID: (Human) 5292

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功能

protein serine/threonine kinase activity protein binding ATP binding transcription factor binding manganese ion binding ribosomal small subunit binding non-receptor serine/threonine protein kinase

参与通路

protein phosphorylation apoptotic process cell cycle multicellular organism development cell proliferation hyaluronan metabolic process negative regulation of apoptotic process negative regulation of sequence-specific DNA binding transcription factor activity protein autophosphorylation vitamin D receptor signaling pathway

PIM1 Monoclonal Antibody (ZP003)

PIM1 Antibody (39-4600) in WB

产品信息

39-4600

免疫印迹 (WB)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

染色质免疫沉淀实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

PIM1 Monoclonal Antibody (ZP003)

PIM1 Antibody (39-4600) in WB

产品信息

39-4600

免疫印迹 (WB)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

染色质免疫沉淀 实验 (ChIP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)