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PKR Antibody (MA5-55327) in WB
Western Blot analysis of various lysates using PKR Recombinant Monoclonal Antibody (Product # MA5-55327) at 1:1,000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
产品信息
MA5-55327
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
5B7B6
抗原
A synthetic peptide corresponding to a sequence within amino acids 450-551 of human PKR/EIF2AK2.
偶联物
形式
Liquid
浓度
0.29 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
产品详细信息
Immunogen Sequence: GTLRYMSPEQ ISSQDYGKEV DLYALGLILA ELLHVCDTAF ETSKFFTDLR DGIISDIFDK KEKTLLQKLL SKKPEDRPNT SEILRTLTVW KKSPEKNERH TC
靶标信息
EIF2AK2 (PKR) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses. EIF2AK2 plays a key role in antiviral defense mediated through its direct activation by double-stranded RNA produced by viral infection. PKR plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth & differentiation. PKR induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival & cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, & PDGF signaling pathway. PKR has been implicated in tumor suppression & malignancy. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: double stranded RNA activated protein kinase; EC 2.7.11.1; eIF-2A protein kinase 2; eIF2a Kinase; Eukaryotic translation initiation factor 2-alpha kinase 2; Interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; MGC126524; OTTHUMP00000201320; P1/eIF-2A protein kinase; p68 kinase; PKR; protein kinase R; Protein kinase RNA-activated; protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; Tyrosine-protein kinase EIF2AK2
基因别名: EIF2AK1; EIF2AK2; PKR; PPP1R83; PRKR
UniProt ID: (Human) P19525
Entrez Gene ID: (Human) 5610
double-stranded RNA binding
protein kinase activity
protein serine/threonine kinase activity
eukaryotic translation initiation factor 2alpha kinase activity
non-membrane spanning protein tyrosine kinase activity
protein binding
ATP binding
protein phosphatase type 2A regulator activity
poly(A) RNA binding
non-receptor serine/threonine protein kinase
activation of MAPKK activity
positive regulation of cytokine production
transcription, DNA-templated
translation
protein phosphorylation
negative regulation of cell proliferation
response to mechanical stimulus
response to virus
response to toxic substance
regulation of translational initiation by eIF2 alpha phosphorylation
negative regulation of translation
peptidyl-tyrosine phosphorylation
evasion or tolerance by virus of host immune response
endoplasmic reticulum unfolded protein response
response to lipopolysaccharide
positive regulation of chemokine production
positive regulation of stress-activated MAPK cascade
response to vitamin E
negative regulation of osteoblast proliferation
regulation of protein phosphatase type 2A activity
cellular response to amino acid starvation
response to interferon-alpha
positive regulation of apoptotic process
negative regulation of apoptotic process
response to exogenous dsRNA
negative regulation of viral genome replication
innate immune response
protein autophosphorylation
positive regulation of NF-kappaB transcription factor activity
defense response to virus
regulation of NLRP3 inflammasome complex assembly
positive regulation of NIK/NF-kappaB signaling
regulation of hematopoietic progenitor cell differentiation
regulation of hematopoietic stem cell proliferation
regulation of hematopoietic stem cell differentiation
