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PLAT Antibody (PA5-106932) in ICC/IF
Immunocytochemistry analysis of PLAT in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with PLAT polyclonal antibody (Product # PA5-106932) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
产品信息
PA5-106932
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human PLAT(Accession P00750), corresponding to amino acid residues L446-T496.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2854596
产品详细信息
Antibody detects endogenous levels of total PLAT.
靶标信息
TPA converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physio pathological events. It specifically cleaves the Arg- -Val bond in plasminogen to form plasmin, and is comprised of a heterodimer of chain A and chain B held by a disulfide bond. TPA binds to fibrin with high affinity. This interaction leads to an increase in the catalytic efficiency of the enzyme between 100-and 1000-fold, due to an increase in affinity for plasminogen. Similarly, binding to heparin increases the activation of plasminogen. Binding to laminin and fibronectin has also been demonstrated. TPA also binds to mannose receptor and the low-density lipoprotein receptor-related protein (LRP1). These proteins are involved in TPA clearance. TPA binds to annexin II and to cytokeratin 8. As yet unidentified interactions on endothelial cells and vascular smooth muscle cells (VSMC) lead to a 100-fold stimulation of plasminogen activation. In addition, binding to VSMC reduces TPA inhibition by PAI-1 by 30-fold.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 3.4.21.68; alteplase; plasminogen/activator kringle; reteplase; t plasminogen activator; t-PA; t-plasminogen activator; tissue plasminogen activator; Tissue-type plasminogen activator
基因别名: AU020998; AW212668; D8Ertd2e; PATISS; PLAT; T-PA; TPA
UniProt ID: (Human) P00750, (Mouse) P11214, (Rat) P19637
Entrez Gene ID: (Human) 5327, (Mouse) 18791, (Rat) 25692
response to hypoxia
cellular protein modification process
proteolysis
blood coagulation
smooth muscle cell migration
plasminogen activation
fibrinolysis
negative regulation of proteolysis
platelet-derived growth factor receptor signaling pathway
synaptic transmission, glutamatergic
positive regulation of ovulation
wound healing
response to peptide hormone
regulation of synaptic plasticity
response to cAMP
cellular response to dexamethasone stimulus
