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产品信息
PA5-22396
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 25 and 251 (Q8TCS8) of PNPase
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Antigen affinity chromatography
保存液
0.1M tris glycine, pH 7, with 20% glycerol
内含物
0.01% thimerosal
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_11152894
产品详细信息
Recommended positive controls: 293T, A431, Jurkat, Raji, mouse liver.
Predicted reactivity: Human (99%), Mouse (93%), Rat (95%), Pig (94%), Bovine (93%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
PNPT1 (polyribonucleotide nucleotidyltransferase 1, mitochondirla) is a RNA-binding protein implicated in numerous RNA metabolic processes. It catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. PNPT1 is a component of the mitochondrial degradosome (mtEXO) complex that degrades 3' overhang double-stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. It is required for correct processing and polyadenylation of mitochondrial mRNAs. It plays a role as a cytoplasmic RNA import factor, in mitochondrial morphogenesis and respiration, regulation of expression of the electron transport chain, regulation of the stability of specific mature miRNAs in melanoma cells, and RNA cell surveilance. Mutations of the gene can result in combined oxidative phosphorylation deficiency 13 (COXPD13) and deafness, autosomal recessive, 70 (DFNB70).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: 3'-5' RNA exonuclease OLD35; DKFZp762K1914; old 35; PNPase 1; PNPase old-35; Polynucleotide phosphorylase 1; Polynucleotide phosphorylase-like protein; Polyribonucleotide nucleotidyltransferase 1, mitochondrial
基因别名: 1200003F12Rik; COXPD13; DFNB70; old-35; OLD35; PNPASE; PNPT1; Pnptl1
UniProt ID: (Human) Q8TCS8, (Mouse) Q8K1R3
Entrez Gene ID: (Human) 87178, (Mouse) 71701
3'-5'-exoribonuclease activity
polyribonucleotide nucleotidyltransferase activity
protein binding
poly(U) RNA binding
poly(G) binding
miRNA binding
poly(A) RNA binding
nucleic acid binding
RNA binding
nuclease activity
exonuclease activity
transferase activity
nucleotidyltransferase activity
hydrolase activity
nucleotidyltransferase
mitochondrial RNA catabolic process
mitochondrial mRNA catabolic process
positive regulation of mitochondrial RNA catabolic process
mitochondrial RNA 5'-end processing
mitochondrial RNA 3'-end processing
RNA catabolic process
mRNA catabolic process
cellular response to oxidative stress
cellular response to interferon-beta
RNA import into mitochondrion
rRNA import into mitochondrion
regulation of cellular respiration
RNA polyadenylation
negative regulation of growth
protein homooligomerization
positive regulation of mRNA catabolic process
protein homotrimerization
mitochondrion morphogenesis
nuclear polyadenylation-dependent mRNA catabolic process
mitotic cell cycle arrest
RNA phosphodiester bond hydrolysis, exonucleolytic
mitochondrial mRNA polyadenylation
positive regulation of miRNA catabolic process
regulation of cellular senescence
RNA processing
mRNA processing
transport
