Invitrogen

PP1 beta Polyclonal Antibody

PP1 beta Antibody in Immunocytochemistry (ICC/IF)

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PP1 beta Antibody (PA1-12379) in ICC/IF

Immunofluorescence analysis of PPP1CB was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.01% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PP1 beta Polyclonal Antibody (Product # PA1-12379) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790) ... View More

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产品信息

PA1-12379

应用

建议稀释比

已发表文章

免疫印迹 (WB)

5-10 µg/mL

- -

免疫细胞化学 (ICC/IF)

1:100

免疫沉淀 (IP)

10-15 µg/mL

产品规格

种属反应

Bovine, Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

C-terminal peptide (amino acids 318-327) of PP1 beta catalytic subunit

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Ammonium sulfate precipitation

保存液

PBS

内含物

0.08% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2170088

产品详细信息

This antibody will not cross-react with other PP1 isoforms.

靶标信息

The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: epididymis secretory sperm binding protein Li 80p; MGC21657; PP-1B; PP2C-beta-X; PP2CB; PP2CBETA; PPC2BETAX; protein phosphatase 1, catalytic subunit, beta isoform; protein phosphatase 1, catalytic subunit, beta isozyme; protein phosphatase 1, catalytic subunit, delta isoform; protein phosphatase 1-beta; protein phosphatase 1-delta; protein phosphatase-1a; serine/threonine protein phosphatase PP1-beta catalytic subunit; Serine/threonine-protein phosphatase PP1-beta catalytic subunit

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基因别名: 1200010B19; HEL-S-80p; PP-1B; PP1B; PP1beta; PPP1CB; PPP1CD