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Invitrogen
PP2A alpha Monoclonal Antibody (7A6)
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
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PP2A alpha Antibody (MA5-18060) in ICC/IF
Immunofluorescence analysis of PP2A alpha was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PP2A alpha Monoclonal Antibody (7A6) (Product # MA5-18060) at 1:100 dilution in 0.1% BSA, incubated at 4 degree ... View More
产品信息
MA5-18060
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat,
Yeast
已发表种属
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
7A6
抗原
C-terminal peptide of human PP2A catalytic subunit (Immunogen sequence: 302-RRTPDYFL-309).
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS
内含物
0.1% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_2539443
产品详细信息
This antibody recognizes the C-terminal of PP2A, both alpha and beta isoforms, in human, mouse, rat and S.cerevisiae samples.
靶标信息
In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2A alpha and PP2A beta. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: B55-GAMMA; IMYPNO; Methyl-PPP2CA; MGC33570; PP2A alpha; PP2A C terminal; PP2A L9; PP2A Leucine 9; PP2A-alpha; PR52; PR55G; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2a, catalytic subunit, alpha isoform; protein phosphatase-2A-alpha; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
基因别名: PP2A; Pp2a1; PP2Ac; PP2CA; PP2Calpha; PPP2CA; R75353; RP-C
UniProt ID: (Human) P67775, (Mouse) P63330, (Rat) P63331
Entrez Gene ID: (Human) 5515, (Mouse) 19052, (Rat) 24672
phosphoprotein phosphatase activity
protein binding
protein C-terminus binding
metal ion binding
GABA receptor binding
protein serine/threonine phosphatase activity
hydrolase activity
enzyme binding
protein kinase binding
protein phosphatase binding
protein domain specific binding
protein complex binding
protein kinase B binding
ion channel binding
protein dimerization activity
tau protein binding
protein phosphatase 2A binding
protein antigen binding
protein heterodimerization activity
protein phosphatase
nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
inactivation of MAPK activity
regulation of DNA replication
regulation of transcription, DNA-templated
protein dephosphorylation
ceramide metabolic process
apoptotic process
mitotic nuclear envelope reassembly
mesoderm development
RNA splicing
response to organic substance
negative regulation of epithelial to mesenchymal transition
second-messenger-mediated signaling
regulation of Wnt signaling pathway
regulation of cell adhesion
negative regulation of cell growth
regulation of growth
negative regulation of tyrosine phosphorylation of Stat3 protein
regulation of cell differentiation
meiotic cell cycle
positive regulation of protein serine/threonine kinase activity
negative regulation of transcription from RNA polymerase II promoter
regulation of protein phosphorylation
negative regulation of protein phosphorylation
regulation of receptor activity
regulation of protein autophosphorylation
positive regulation of protein phosphatase type 2A activity
positive regulation of protein dephosphorylation
regulation of protein catabolic process
negative regulation of transcription factor import into nucleus
positive regulation of apoptotic process
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
regulation of cell cycle
protein heterotrimerization
cellular response to glucose stimulus
negative regulation of calcium ion transmembrane transporter activity
cardiac ventricle development
heart development
cellular response to insulin stimulus
cellular response to calcium ion
cellular response to cytokine stimulus
cellular response to ethanol
cellular response to follicle-stimulating hormone stimulus
cellular response to steroid hormone stimulus
