Invitrogen

PPT1 Monoclonal Antibody (10F3)

PPT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PPT1 Antibody (MA5-49231) in IHC (P)

Immunohistochemistry analysis of PPT1 in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with PPT1 Monoclonal antibody (Product # MA5-49231) using a dilution of 1 μg/mL overnight at 4°C. Biotinylated goat anti-mouse IgG was... View More

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产品信息

MA5-49231

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

10F3

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human PPT1 (191-224aa KEDVYRNHSIFLADINQERGINESYKKNLMALKK).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_3074889

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Immunogen sequence is different from the related mouse and rat sequences by four amino acids.

靶标信息

The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。