Invitrogen

PRDM5 Polyclonal Antibody

查看其他6个PRDM5抗体

抗体检测数据 (5)

PRDM5 Antibody in Immunocytochemistry (ICC/IF)

PRDM5 Antibody in Immunocytochemistry (ICC/IF)

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PRDM5 Antibody (PA5-96653) in ICC/IF

Immunofluorescence analysis of PRDM5 in A549 cells. Samples were incubated with PRDM5 Polyclonal antibody (Product # PA5-96653). Blue: DAPI for nuclear staining.

PRDM5 Antibody in Immunocytochemistry (ICC/IF)

PRDM5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRDM5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRDM5 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PRDM5 Antibody in Western Blot (WB)

Product Image Gallery

PRDM5 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PRDM5 in A549 cells. Samples were incubated with PRDM5 Polyclonal antibody (Product # PA5-96653). Blue: DAPI for nuclear staining.

PRDM5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PRDM5 in paraffin-embedded mouse spleen. Samples were incubated with PRDM5 Polyclonal antibody (Product # PA5-96653) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

PRDM5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PRDM5 in paraffin-embedded mouse liver. Samples were incubated with PRDM5 Polyclonal antibody (Product # PA5-96653) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

PRDM5 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of PRDM5 in paraffin-embedded rat spleen. Samples were incubated with PRDM5 Polyclonal antibody (Product # PA5-96653) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

PRDM5 Antibody in Western Blot (WB) — Western blot analysis of PRDM5 in extracts of various cell lines. Samples were incubated with PRDM5 Polyclonal antibody (Product # PA5-96653) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

产品信息

PA5-96653

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PRDM5 (NP_0611692)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.38 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2808455

产品详细信息

Immunogen sequence: MLGMYVPDRF SLKSSRVQDG MGLYTARRVR KGEKFGPFAG EKRMPEDLDE NMDYRLMWEV RGSKGEVLYI LDATNPRHSN WLRFVHEAPS QEQKNLAAIQ; Positive Samples: Mouse kidney, Mouse heart, Mouse lung, Mouse ovary, Rat kidney, Rat liver; Cellular Location: Nucleus

靶标信息

PRDM5, a novel member of PR domain protein family, contains a PR-domain at the -NH2 terminus followed by 16 zinc finger motifs. It is found to have a role in cell differentiation and tumorigenesis. Reports suggest PRDM5 causes G2/M arrest and apoptosis upon infection of tumor cells and has been found to be silenced in breast, ovarian and lung cancers, suggesting a potential clinical significance.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: PR domain containing 5; PR domain zinc finger protein 5; PR domain-containing protein 5

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基因别名: 4432417F03Rik; 6530401I24Rik; AI197291; BCS2; E130112L17Rik; PFM2; PRDM5

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UniProt ID: (Human) Q9NQX1, (Mouse) Q9CXE0

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Entrez Gene ID: (Human) 11107, (Mouse) 70779, (Rat) 689788

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RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding protein binding methyltransferase activity sequence-specific DNA binding transcription regulatory region DNA binding metal ion binding repressing transcription factor binding rRNA (adenine-N6,N6-)-dimethyltransferase activity transcription regulatory region sequence-specific DNA binding nucleic acid binding DNA binding protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity C2H2 zinc finger transcription factor

参与通路

negative regulation of transcription from RNA polymerase II promoter mitotic cell cycle transcription, DNA-templated histone deacetylation negative regulation of transcription, DNA-templated histone H3-K9 methylation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter chromatin modification methylation