Invitrogen

PSMD7 Polyclonal Antibody

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 篇参考文献

查看其他10个PSMD7抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (4)
高级验证 (1)

PSMD7 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

PSMD7 Antibody (PA1-1963) in ICC/IF

Immunofluorescence analysis of PSMD7 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PSMD7 Rabbit Polyclonal Antibody (Product # PA1-1963) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More

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Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with PSMD7 siRNA and loss of signal was observed in Western Blot using Anti-PSMD7 Antibody (Product # PA1-1963). {KD}

产品信息

PA1-1963

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-2 µg/mL

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

产品规格

种属反应

Hamster, Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues A(55) V P F D E D D K D D S(66) C of human 19S subunit S12.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2170800

产品详细信息

PA1-1963 detects proteasome 19S subunit S12 from human, rat, mouse and hamster cell lysates.

PA1-1963 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~37 kDa protein representing proteasome 19S subunit S12 from human, rat, mouse, and hamster cell extracts. An additional unknown band is detected at ~48 kDa in some cell lysates.

PA1-1963 immunogen is a synthetic peptide corresponding to residues A(55) V P F D E D D K D D S(66) C of human 19S subunit S12. The immunizing peptide sequence is 100% conserved in Danio rerio and 91% conserved in Drosphila, C. elegans and Anopheles. PA1-1963 immunizing peptide (Cat. # PEP-211) is available for use in neutralization and control experiments.

靶标信息

Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signaling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (approximately 31), barrel-shaped molecular machine with an apparent molecular weight of approximately 2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Interferon β induces clearance of mutant ataxin 7 and improves locomotion in SCA7 knock-in mice.

Brain : a journal of neurology

Chort A,Alves S,Marinello M,Dufresnois B,Dornbierer JG,Tesson C,Latouche M,Baker DP,Barkats M,El Hachimi KH,Ruberg M,Janer A,Stevanin G,Brice A,Sittler A

PA1-1963 was used in western blot to study the ability of IFNbeta to induce PML-dependent ataxin 7 clearance and improve motor function in a murine model of spinocerebellar ataxia 7

Sat Jun 01 00:00:00 EDT 2013

Persistent hijacking of brain proteasomes in HIV-associated dementia.

The American journal of pathology

Nguyen TP,Soukup VM,Gelman BB

PA1-1963 was used in western blot to study the effect of HIV infection on brain proteasomes and its role in dementia

Mon Feb 01 00:00:00 EST 2010

生物信息学

蛋白别名: 26S proteasome non-ATPase regulatory subunit 7; 26S proteasome regulatory subunit RPN8; 26S proteasome regulatory subunit S12; Moloney leukemia virus-34 proviral integration; Mov34 homolog; Mov34 protein; Mov34 protein homolog; proteasome (prosome, macropain) 26S subunit, non-ATPase, 7; proteasome (prosome, macropain) 26S subunit, non-ATPase, 7 (Mov34 homolog); Proteasome subunit p40

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基因别名: AW107203; Mov-34; MOV34; MOV34L; P40; PSMD7; Rpn8; S12

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UniProt ID: (Human) P51665, (Mouse) P26516