Invitrogen

Pan SHANK Monoclonal Antibody (N23b/49), FITC

Pan SHANK Antibody in Immunocytochemistry (ICC/IF)

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Pan SHANK Antibody (MA5-45439) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with SHANK (pan) monoclonal antibody (Product # MA5-45439) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain used was Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1,000, 1:5,000 ... View More

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产品信息

MA5-45439

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

免疫组化 (IHC)

1:1,000

免疫细胞化学 (ICC/IF)

1:100

免疫沉淀 (IP)

Assay-dependent

Antibody Microarray (AM)

Assay-dependent

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

N23b/49

抗原

Fusion protein amino acids 84-309 of rat Shank2

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偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine

内含物

no preservative

保存条件

4° C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2931893

产品详细信息

1 µg/mL of MA5-45439 was sufficient for detection of Shank1-4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 160kDa. Recognizes Shank1, 2 and 3.

靶标信息

Shank proteins make up a family of scaffold proteins identified through their interaction with a variety of membrane and cytoplasmic proteins. Shank proteins at postsynaptic sites of excitatory synapses play roles in signal transmission into the postsynaptic neuron. Studies suggest that Shank2 is expressed in the neurons of the developing retina, and could play a role in the neuronal differentiation of the developing retina. Other recent studies suggest that the disruption of glutamate receptors at the Shank postsynaptic platform could contribute to the destruction of the postsynaptic density, which underlies the synaptic dysfunction and loss in Alzheimer's disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: cortactin SH3 domain-binding protein; Cortactin-binding protein 1; CortBP1; GKAP/SAPAP interacting protein; GKAP/SAPAP-interacting protein; proline rich synapse associated protein 1; proline rich synapse associated protein 2; proline-rich synapse associated protein 1; Proline-rich synapse-associated protein 1; Proline-rich synapse-associated protein 2; ProSAP1; ProSAP2; SAPAP-interacting protein synamon; SH3 and multiple ankyrin repeat domains 1; SH3 and multiple ankyrin repeat domains protein 1; SH3 and multiple ankyrin repeat domains protein 2; SH3 and multiple ankyrin repeat domains protein 3; SH3 and multiple ankyrin repeat domains protein 3 variant c-3; SH3 and multiple ankyrin repeat domains protein 3 variant c-4; SH3 and multiple ankyrin repeat domains-like protein 3 variant 1; SH3 and multiple ankyrin repeat domains-like protein 3 variant 2; SH3 and multiple ankyrin repeat domains-like protein 3 variant 3; SH3 and multiple ankyrin repeat domains-like protein 3 variant c-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant c-2; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-2; SH3 and multiple ankyrin repeat domains-like protein 3 variant d-3; SH3 and multiple ankyrin repeat domains-like protein 3 variant e-1; SH3 and multiple ankyrin repeat domains-like protein 3 variant e-2; SH3/ankyrin domain gene 1; SH3/ankyrin domain gene 2; SH3/ankyrin domain gene 3; shank postsynaptic density protein; Shank postsynaptic density protein 3a; Shank1; Shank2; Shank3; shank3 splice variant II; shank3 splice variant III; shank3 splice variant IV; shank3e splice variant III; shank3e splice variant IV; Somatostatin receptor-interacting protein; SPANK-1; SPANK-2; SPANK-3; SSTR-interacting protein; Synamon

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基因别名: AI841104; AUTS17; CORTBP1; CTTNBP1; DEL22q13.3; KIAA1022; Kiaa1650; mKIAA1022; PROSAP1; PROSAP2; PSAP2; SCZD15; SHANK; SHANK1; Shank1a; SHANK2; Shank3; Shank3b; SHANK3c-3; SHANK3c-4; SPANK-1; SPANK-2; SPANK-3; Spank1; SSTRIP; synamon