Invitrogen

Parkin Polyclonal Antibody

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抗体检测数据 (2)

Parkin Antibody in Western Blot (WB)

Parkin Antibody in Western Blot (WB)

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Parkin Antibody (PA1-4552) in WB

Western blot analysis of Parkin using a polyclonal antibody (Product # PA1-4552).

Parkin Antibody in Western Blot (WB)

Parkin Antibody in Western Blot (WB)

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Parkin Antibody in Western Blot (WB) — Western blot analysis of Parkin using a polyclonal antibody (Product # PA1-4552).

Parkin Antibody in Western Blot (WB) — Western blot analysis of Parkin using a polyclonal antibody (Product # PA1-4552).

产品信息

PA1-4552

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:250-1:1,000

-

免疫沉淀 (IP)

1:250-1:1,000

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Internal sequence of the 52 kDa Parkin human protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_1077464

产品详细信息

PA1-4552 detects Parkin in human samples.

PA1-4552 has been successfully used in Western blotting and immunoprecipitation procedures. By Western blot, this antibody detects a ~52 kDa protein representing Parkin in HEK cells stably expressing the protein.

PA1-4552 immunizing peptide corresponds to an internal sequence of the 52 kDa Parkin human protein.

Maintain antibody at 4ºC for up to 3 months. For long term storage, store at -20ºC, avoiding freeze/thaw cycles.

靶标信息

The precise function of Parkin gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: E3 ubiquitin ligase; E3 ubiquitin-protein ligase parkin; OTTHUMP00000017562; OTTHUMP00000017563; OTTHUMP00000017564; Parkin; parkin 2; Parkin isoform 1; Parkin RBR E3 ubiquitin-protein ligase; Parkinson disease (autosomal recessive, juvenile) 2, parkin; Parkinson disease protein 2; Parkinson juvenile disease protein 2; parkinson protein 2 E3 ubiquitin protein ligase; parkinson protein 2, E3 ubiquitin protein ligase (parkin)

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基因别名: AR-JP; LPRS2; PARK2; PDJ; PRKN

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UniProt ID: (Human) O60260

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Entrez Gene ID: (Human) 5071

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transcription regulatory region sequence-specific DNA binding G-protein coupled receptor binding transcription factor activity, sequence-specific DNA binding actin binding ubiquitin-protein transferase activity protein binding beta-catenin binding zinc ion binding tubulin binding ligase activity SH3 domain binding enzyme binding kinase binding protein kinase binding PDZ domain binding Hsp70 protein binding heat shock protein binding ubiquitin conjugating enzyme binding ubiquitin protein ligase binding identical protein binding histone deacetylase binding ubiquitin binding phospholipase binding chaperone binding ubiquitin protein ligase activity cullin family protein binding ubiquitin protein ligase activity involved in ERAD pathway ubiquitin-specific protease binding F-box domain binding ubiquitin-protein ligase

参与通路

negative regulation of transcription from RNA polymerase II promoter protein polyubiquitination mitochondrial fission mitophagy negative regulation of protein phosphorylation startle response transcription, DNA-templated protein monoubiquitination response to oxidative stress mitochondrion organization central nervous system development learning adult locomotory behavior proteasomal protein catabolic process regulation of autophagy positive regulation of gene expression negative regulation of gene expression positive regulation of mitochondrial fusion negative regulation of mitochondrial fusion regulation of mitochondrion organization regulation of glucose metabolic process free ubiquitin chain polymerization regulation of dopamine secretion macroautophagy protein ubiquitination regulation of protein ubiquitination protein destabilization negative regulation of actin filament bundle assembly regulation of lipid transport positive regulation of proteasomal ubiquitin-dependent protein catabolic process negative regulation of glucokinase activity cellular response to unfolded protein response to endoplasmic reticulum stress synaptic transmission, glutamatergic protein K29-linked ubiquitination ERAD pathway regulation of dopamine metabolic process norepinephrine metabolic process dopamine metabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of DNA binding negative regulation of neuron apoptotic process cellular protein catabolic process cellular protein metabolic process protein K27-linked ubiquitination negative regulation by host of viral genome replication positive regulation of protein catabolic process positive regulation of transcription from RNA polymerase II promoter negative regulation of JNK cascade negative regulation of insulin secretion protein stabilization positive regulation of neurotransmitter uptake dopamine uptake involved in synaptic transmission protein autoubiquitination regulation of mitochondrial membrane potential zinc ion homeostasis negative regulation of cell death regulation of canonical Wnt signaling pathway parkin-mediated mitophagy in response to mitochondrial depolarization neuron cellular homeostasis protein K63-linked ubiquitination protein localization to mitochondrion aggresome assembly protein K48-linked ubiquitination protein K11-linked ubiquitination cellular response to manganese ion protein K6-linked ubiquitination negative regulation of canonical Wnt signaling pathway positive regulation of mitochondrial fission negative regulation of release of cytochrome c from mitochondria cellular response to toxic substance mitophagy in response to mitochondrial depolarization regulation of cellular response to oxidative stress negative regulation of neuron death positive regulation of proteasomal protein catabolic process negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator negative regulation of primary amine oxidase activity positive regulation of protein linear polyubiquitination regulation of synaptic vesicle transport negative regulation of oxidative stress-induced cell death regulation of protein targeting to mitochondrion positive regulation of tumor necrosis factor-mediated signaling pathway cellular response to dopamine negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway negative regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway positive regulation of dendrite extension negative regulation of spontaneous neurotransmitter secretion regulation of reactive oxygen species metabolic process negative regulation of reactive oxygen species metabolic process