Invitrogen

Parkin Recombinant Rabbit Monoclonal Antibody (21H24L9)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

5个已发表图片
4 篇参考文献
查看其他25个Parkin抗体
说明书
实验方案
技术支持
说明书
实验方案
技术支持

产品信息

702785

应用
建议稀释比
已发表文章

免疫印迹 (WB)

2.5 µg/mL
查看4篇已发表文章 4篇已发表文章

免疫沉淀 (IP)

Assay-dependent
-
产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

21H24L9

抗原

Protein corresponding to human Parkin [aa1-aa465]
查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2724937

产品详细信息

This antibody is predicted to react with Monkey, Rat, Pig, Mouse.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

The precise function of Parkin gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: E3 ubiquitin ligase; E3 ubiquitin-protein ligase parkin; OTTHUMP00000017562; OTTHUMP00000017563; OTTHUMP00000017564; Parkin; parkin 2; Parkin isoform 1; parkin protein; Parkin RBR E3 ubiquitin-protein ligase; parkin variant SV5DEL; Parkinson disease (autosomal recessive, juvenile) 2, parkin; Parkinson disease protein 2; Parkinson juvenile disease protein 2; parkinson protein 2 E3 ubiquitin protein ligase; parkinson protein 2, E3 ubiquitin protein ligase; parkinson protein 2, E3 ubiquitin protein ligase (parkin)

查看更多 收起

基因别名: AR-JP; LPRS2; Park; PARK2; PDJ; PRKN

查看更多 收起

UniProt ID: (Human) O60260, (Rat) Q9JK66, (Mouse) Q9WVS6

查看更多 收起

Entrez Gene ID: (Human) 5071, (Rat) 56816, (Mouse) 50873

查看更多 收起

功能
transcription regulatory region sequence-specific DNA binding G-protein coupled receptor binding transcription factor activity, sequence-specific DNA binding actin binding ubiquitin-protein transferase activity protein binding beta-catenin binding zinc ion binding tubulin binding ligase activity SH3 domain binding enzyme binding kinase binding protein kinase binding PDZ domain binding Hsp70 protein binding heat shock protein binding ubiquitin conjugating enzyme binding ubiquitin protein ligase binding identical protein binding histone deacetylase binding ubiquitin binding phospholipase binding chaperone binding ubiquitin protein ligase activity cullin family protein binding ubiquitin protein ligase activity involved in ERAD pathway ubiquitin-specific protease binding F-box domain binding protein complex binding UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity metal ion binding protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase
参与通路
negative regulation of transcription from RNA polymerase II promoter protein polyubiquitination mitochondrial fission mitophagy negative regulation of protein phosphorylation startle response transcription, DNA-templated protein monoubiquitination response to oxidative stress mitochondrion organization central nervous system development learning adult locomotory behavior proteasomal protein catabolic process regulation of autophagy positive regulation of gene expression negative regulation of gene expression positive regulation of mitochondrial fusion negative regulation of mitochondrial fusion regulation of mitochondrion organization regulation of glucose metabolic process free ubiquitin chain polymerization regulation of dopamine secretion macroautophagy protein ubiquitination regulation of protein ubiquitination protein destabilization negative regulation of actin filament bundle assembly regulation of lipid transport positive regulation of proteasomal ubiquitin-dependent protein catabolic process negative regulation of glucokinase activity cellular response to unfolded protein response to endoplasmic reticulum stress synaptic transmission, glutamatergic protein K29-linked ubiquitination ERAD pathway regulation of dopamine metabolic process norepinephrine metabolic process dopamine metabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of DNA binding negative regulation of neuron apoptotic process cellular protein catabolic process cellular protein metabolic process protein K27-linked ubiquitination negative regulation by host of viral genome replication positive regulation of protein catabolic process positive regulation of transcription from RNA polymerase II promoter negative regulation of JNK cascade negative regulation of insulin secretion protein stabilization positive regulation of neurotransmitter uptake dopamine uptake involved in synaptic transmission protein autoubiquitination regulation of mitochondrial membrane potential zinc ion homeostasis negative regulation of cell death regulation of canonical Wnt signaling pathway parkin-mediated mitophagy in response to mitochondrial depolarization neuron cellular homeostasis protein K63-linked ubiquitination protein localization to mitochondrion aggresome assembly protein K48-linked ubiquitination protein K11-linked ubiquitination cellular response to manganese ion protein K6-linked ubiquitination negative regulation of canonical Wnt signaling pathway positive regulation of mitochondrial fission negative regulation of release of cytochrome c from mitochondria cellular response to toxic substance mitophagy in response to mitochondrial depolarization regulation of cellular response to oxidative stress negative regulation of neuron death positive regulation of proteasomal protein catabolic process negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway by p53 class mediator negative regulation of primary amine oxidase activity positive regulation of protein linear polyubiquitination regulation of synaptic vesicle transport negative regulation of oxidative stress-induced cell death regulation of protein targeting to mitochondrion positive regulation of tumor necrosis factor-mediated signaling pathway cellular response to dopamine negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway positive regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway negative regulation of endoplasmic reticulum stress-induced neuron intrinsic apoptotic signaling pathway positive regulation of dendrite extension negative regulation of spontaneous neurotransmitter secretion regulation of reactive oxygen species metabolic process negative regulation of reactive oxygen species metabolic process synaptic transmission, dopaminergic cellular protein modification process ubiquitin-dependent protein catabolic process response to unfolded protein locomotory behavior protein metabolic process response to drug neuron maturation regulation of neurotransmitter secretion modulation of synaptic transmission regulation of protein transport response to corticosterone mitochondrion localization negative regulation of oxidative stress-induced neuron death regulation of transcription, DNA-templated autophagy
guarantee_icon

性能保障

只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符，您即可享受免费退换货服务。*

了解更多
help_icon

随时为您服务

获取常见问题的专家解答，或者直接联系我们的技术专家，咨询应用、设备、常规产品使用问题。

联系技术支持