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Peroxidase Antibody (200-3138-0100) in WB
Western Blot of Mouse anti-Peroxidase (Horseradish) Antibody. Lane 1: Peroxidase (Horseradish). Load: 100 µg per lane. Primary antibody: Mouse anti-Peroxidase (Horseradish) Antibody at 1:1,000 overnight at 4°C. Secondary antibody: Fluorescein conjugated mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 44 kDa, 44 kDa for Horseradish Peroxidase.
产品信息
200-3138-0100
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
宿主/亚型
Mouse
分类
Polyclonal
类型
Antibody
抗原
Peroxidase [Horseradish]
偶联物
形式
Lyophilized
浓度
1 mg/mL
纯化类型
Ion-exchange chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Anti-Peroxidase (horseradish) (Mouse) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum as well as purified and partially purified Peroxidase [Horseradish]. Cross reactivity against Peroxidase from other tissues and species may occur but have not been specifically determined.
靶标信息
HRP, also known as horseradish peroxidase, is an enzyme that is commonly used in biochemistry and molecular biology research. It is derived from the root of the horseradish plant and catalyzes the oxidation of a wide range of substrates in the presence of hydrogen peroxide. HRP is widely used as a detection enzyme in immunoassays, such as ELISAs and Western blots, due to its high sensitivity and stability. It can also be used as a tracer in enzyme-linked immunosorbent assays (ELISAs) and as a labeling agent in fluorescent microscopy.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Horseradish Peroxidase
