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Phospho-APC6 (Thr580) Antibody (600-401-876) in WB
Western blot using Rocklands Affinity Purified anti-APC6 pT580 antibody shows detection of a band ~72 kDa corresponding to phosphorylated human APC6 (arrowhead lane 1). Lane 1 - nocodazole treated HeLa whole cell lysate. Lane 2 - Reactivity is not seen in lysates from asynchronous HeLa whole cell cultures. Each lane contains approximately 35ug of lysates, separated by 4-20% SDS-PAGE Tris-HEPES and then tra... View More
产品信息
600-401-876
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding pT580 of Human Apc6 protein.
偶联物
形式
Liquid
浓度
0.97 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is specific for phosphorylated human APC6 protein at the pT580 residue. BLAST analysis indicates 100 % homology of the immunizing sequence with APC6 from human. No cross reactivity is expected against homologues from other sources. This antibody is expected to react with all isoforms of this protein. Minimal reactivity is expected with the non-phosphorylated form of the protein.
靶标信息
The protein encoded by this gene is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Anaphase-promoting complex subunit 6; anaphase-promoting complex, subunit 6; APC6; CDC16 homolog; CDC16Hs; cell division cycle 16 homolog; Cell division cycle protein 16 homolog; Cyclosome subunit 6; RP11-569D9.4
基因别名: ANAPC6; APC6; CDC16; CDC16Hs; CUT9
UniProt ID: (Human) Q13042
Entrez Gene ID: (Human) 8881
mitotic nuclear division
regulation of mitotic nuclear division
cell proliferation
anaphase-promoting complex-dependent catabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
proteasome-mediated ubiquitin-dependent protein catabolic process
cell division
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
protein K11-linked ubiquitination
