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Invitrogen
Phospho-CHK1 (Ser345) Polyclonal Antibody
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
图: 1 / 12
Phospho-CHK1 (Ser345) Antibody (PA5-34625) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Phospho-CHK1 (Ser345) using Phospho-CHK1 (Ser345) Polyclonal Antibody (Product # PA5-34625) (Green) diluted at 1:500. HeLa cells mock (left) and treated with 100 J/m2 UVC and recover for 8 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
产品信息
PA5-34625
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic phospho-peptide corresponding to amino acids residues surrounding the phospho Ser345 of CHK1
偶联物
形式
Liquid
浓度
1.66 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol, 1% BSA
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2551977
产品详细信息
Recommended positive controls: HCT116, HCT116 cells with 30uM cisplatin treatment for 24hr.
Predicted reactivity: Mouse (100%), Rat (100%), Bovine (100%).
IHC notes, Requires antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
CHEK1 (CHK1) is a serine/threonine protein kinase involved in preventing replication when DNA integrity is compromised. CHK1 is a key mediator in the DNA damage-induced checkpoint network. CHK1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. When the G2 or S checkpoint is abrogated by the inhibition of CHK1, p53-deficient cancer cells undergo mitotic catastrophe and eventually apoptosis, whereas normal cells are still arrested in the G1 phase. Thus, CHK1 inhibitors can preferentially potentiate the efficacy of DNA damaging agents in cancer cells, and Chk1 is an attractive therapeutic target for cancer treatment, especially since approximately 50% of all human cancers are p53-deficient.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Cell cycle checkpoint kinase; checkpoint kinase 1 homolog; Checkpoint kinase-1; Checkpoint, S. pombe, homolog of, 1; CHK1 checkpoint homolog; Chk1-S; homolog of Chk1-S; HRD1 Protein; rad27 homolog; Serine/threonine-protein kinase Chk1
基因别名: C85740; CHEK1; CHK1; rad27
UniProt ID: (Human) O14757, (Mouse) O35280
Entrez Gene ID: (Human) 1111, (Mouse) 12649
DNA damage checkpoint
G2/M transition of mitotic cell cycle
DNA replication
DNA repair
cellular response to DNA damage stimulus
DNA damage induced protein phosphorylation
regulation of double-strand break repair via homologous recombination
regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage
peptidyl-threonine phosphorylation
G2 DNA damage checkpoint
histone H3-T11 phosphorylation
regulation of cell proliferation
negative regulation of mitotic nuclear division
regulation of mitotic centrosome separation
chromatin-mediated maintenance of transcription
cellular response to mechanical stimulus
replicative senescence
regulation of signal transduction by p53 class mediator
regulation of histone H3-K9 acetylation
protein phosphorylation
cell cycle
regulation of gene expression
negative regulation of G2/M transition of mitotic cell cycle
phosphorylation
negative regulation of DNA biosynthetic process
