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Invitrogen
Phospho-CREB/ATF1 (Ser133, Ser63) Monoclonal Antibody (10E9)
高级验证
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
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Phospho-CREB/ATF1 (Ser133, Ser63) Antibody (MA1-114) in ICC/IF
Immunofluorescent analysis of Phospho-CREB pSer133 (green) in HeLa cells either left untreated (left panel) or treated with 1uM Camptothecin (right panel) for 20 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-CREB pSer133 mono... View More
产品信息
MA1-114
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Human,
Mouse
已发表种属
Not Applicable
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
10E9
抗原
A synthetic phosphopeptide corresponding to amino acids 125-135 surrounding the Ser133 phosphorylation site of human CREB
偶联物
形式
Lyophilized
浓度
0.1 mg/mL
纯化类型
Size-exclusion chromatography
保存液
PBS, pH 7.2, with sucrose, PEG, 50% glycerol
内含物
0.09% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
运输条件
Wet ice
RRID
AB_2536825
产品详细信息
This antibody recognizes CREB protein phosphorylated at serine 133 and ATF1 phosphorylated at serine 63. The antibody was purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.
Reconstitute with 1 mL H2O (15 min, RT)
Aliquot and freeze in liquid nitrogen. Antibody can be stored frozen at -80ºC for up to 1 year. Thaw aliquots at 37ºC; thawed aliquots may be stored at 4ºC for up to 3 months. Avoid repeated freeze/thaw cycles.
This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
靶标信息
Cyclic AMP response element binding protein (CREB) is a basic/leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44/42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Activating transcription factor 1; active transcription factor CREB; cAMP-dependent transcription factor ATF-1; cAMP-response element-binding protein-1; cAMP-responsive element-binding protein 1; CREB-1; cyclic adenosine 3',5'-monophosphate response element-binding protein CREB; Cyclic AMP-dependent transcription factor ATF-1; Cyclic AMP-responsive element-binding protein 1; Protein TREB36; Ser133; TCR-ATF1; transactivator protein
基因别名: 2310001E10Rik; 3526402H21Rik; ATF1; AV083133; CREB; CREB-1; CREB1; EWS-ATF1; FUS/ATF-1; TREB36
UniProt ID: (Human) P18846, (Mouse) P81269, (Human) P16220, (Mouse) Q01147
Entrez Gene ID: (Dog) 486545, (Human) 466, (Mouse) 11908, (Dog) 607922, (Human) 1385, (Mouse) 12912
RNA polymerase II regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
protein complex binding
protein heterodimerization activity
DNA binding
sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II activating transcription factor binding
transcriptional activator activity, RNA polymerase II transcription factor binding
transcription cofactor activity
enzyme binding
Hsp70 protein binding
histone acetyltransferase binding
cAMP response element binding
identical protein binding
arrestin family protein binding
double-stranded DNA binding
transcription factor binding
transcription regulatory region DNA binding
transcription, DNA-templated
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
protein phosphorylation
transforming growth factor beta receptor signaling pathway
axonogenesis
lactation
memory
circadian rhythm
regulation of cell size
response to organic substance
negative regulation of transcription by competitive promoter binding
pituitary gland development
cell differentiation
mammary gland development
positive regulation of transforming growth factor beta3 production
secretory granule organization
response to glucagon
positive regulation of multicellular organism growth
response to drug
regulation of circadian rhythm
regulation of apoptotic process
positive regulation of fat cell differentiation
positive regulation of osteoclast differentiation
positive regulation of transcription, DNA-templated
positive regulation of RNA polymerase II transcriptional preinitiation complex assembly
positive regulation of transcription from RNA polymerase II promoter
positive regulation of hormone secretion
positive regulation of lipid biosynthetic process
rhythmic process
protein stabilization
lung epithelium development
lung saccule development
Type I pneumocyte differentiation
cellular response to zinc ion
cellular response to growth factor stimulus
regulation of transcription from RNA polymerase II promoter
positive regulation of neuron projection development
cellular protein complex assembly
positive regulation of DNA replication
response to organic cyclic compound
response to cobalt ion
response to hypoxia
signal transduction
axon guidance
aging
visual learning
response to activity
viral process
chemotaxis to arachidonic acid
response to nicotine
cellular response to hepatocyte growth factor stimulus
cellular response to platelet-derived growth factor stimulus
response to antidepressant
positive regulation of apoptotic process
regulation of fibroblast proliferation
regulation of glial cell proliferation
cellular response to fatty acid
positive regulation of long-term synaptic potentiation
negative regulation of neuron death
response to L-glutamate
cellular response to nerve growth factor stimulus
cellular response to insulin-like growth factor stimulus
