Invitrogen

Phospho-CRYAB (Ser45) Polyclonal Antibody

Phospho-CRYAB (Ser45) Antibody in Western Blot (WB)

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Phospho-CRYAB (Ser45) Antibody (PA1-011) in WB

Western blot detection of recombinant bovine phospho-alpha-B crystallin (Ser45) using Product # PA1-011. Lane 1 is phospho-alpha-B crystallin (Ser45) and lane 2 is unphosphorylated alpha-B crystallin.

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产品信息

PA1-011

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5 µg/mL

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免疫组化（石蜡） (IHC (P))

8 µg/mL

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免疫沉淀 (IP)

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产品规格

种属反应

Bovine, Human, Mouse, Rat

已发表种属

Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic phopshopeptide corresponding to residues L(44) S(p) P F Y L R P P S F(54) C of human alpha-B Crystallin.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 30mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2084437

产品详细信息

PA1-011 detects phospho-alpha-B crystallin (Ser45) from bovine, human, mouse and rat samples. This antibody does not detect the unphosphorylated form of the protein.

PA1-011 has been successfully used in Western blot, immunohistochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~20 kDa protein representing recombinant bovine phospho-alpha-B crystallin (Ser45). Immunofluorescence staining of phospho-alpha-B crystallin (Ser45) in U373 MG cells results in cytoplasmic staining.

The PA1-011 immunogen is a synthetic phopshopeptide corresponding to residues L(44) S(p) P F Y L R P P S F(54) C of human alpha-B Crystallin.

Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.

靶标信息

Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Phosphorylation of αB-crystallin supports reactive astrogliosis in demyelination.

Proceedings of the National Academy of Sciences of the United States of America

Kuipers HF,Yoon J,van Horssen J,Han MH,Bollyky PL,Palmer TD,Steinman L

PA1011 was used in immunoprecipitation to determine the role of CRYAB in primary demyelinating events

Tue Feb 28 00:00:00 EST 2017

Hyperglycemia induced expression, phosphorylation, and translocation of αB-crystallin in rat skeletal muscle.

IUBMB life

Reddy VS,Jakhotia S,Reddy PY,Reddy GB

PA1-011 was used in immunohistochemistry - paraffin section and western blot to study the role of alphaBC under chronic hyperglycemia in rat skeletal muscle

Wed Apr 01 00:00:00 EDT 2015

Expression and induction of small heat shock proteins in rat heart under chronic hyperglycemic conditions.

Archives of biochemistry and biophysics

Reddy VS,Kumar ChU,Raghu G,Reddy GB

PA1-011 was used in western blot to examine the expression of sHsp under chronic hyperglycemic conditions in rat heart

Mon Sep 15 00:00:00 EDT 2014

O-GlcNAcylation of αB-crystallin regulates its stress-induced translocation and cytoprotection.

Molecular and cellular biochemistry

Krishnamoorthy V,Donofrio AJ,Martin JL

PA1-011 was used in western blot to study the role of alphaB-crystallin O-GlcNAcylation in regulating its translocation in response to stress and its cytoprotective effects in cardiomyocytes

Mon Jul 01 00:00:00 EDT 2013

生物信息学

蛋白别名: alpha B-crystallin; alpha crystallin B; Alpha(B)-crystallin; Alpha-crystallin B chain; CryAB antibody; crystallin, alpha 2; crystallin, alpha B; crystallin, alpha polypeptide 2; epididymis secretory protein Li 101; Heat shock protein beta-5; heat shock protein CryAB; Heat shock protein family B member 5; heat shock protein HspB5; heat-shock 20 kD like-protein; HspB5; P23; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component

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基因别名: AACRYA; CMD1II; Crya-2; CRYA2; CRYAB; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2; P23