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Invitrogen

Phospho-DNM1L (Ser616) Recombinant Rabbit Monoclonal Antibody (PSH06-77)

Name: Phospho-DNM1L (Ser616) Recombinant Rabbit Monoclonal Antibody (PSH06-77)
Brand: Invitrogen
SKU: MA556582
Price: 4899.0 CNY

查看其他33个DNM1L抗体

说明书

实验方案

技术支持

货号 MA5-56582

CNY 4,899.00

100 µL

下单后预计有货时间 08-Apr-2025

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说明书

实验方案

技术支持

抗体检测数据 (11)

Application

Phospho-DNM1L (Ser616) Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 11

Phospho-DNM1L (Ser616) Antibody (MA5-56582) in ICC/IF

Immunofluorescence analysis of Phospho DNM1L was performed using 70% confluent log phase HEK-293 cells treated with nocodazole at 100 ng/mL for 17 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Phospho-DNM1L (Ser616) Recombinant Rabbit Monoclonal Ant... View More

Phospho-DNM1L (Ser616) Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Phospho-DNM1L (Ser616) Antibody in Western Blot (WB)

Phospho-DNM1L (Ser616) Antibody in Flow Cytometry (Flow)

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产品信息

MA5-56582

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:5,000

免疫组化（石蜡） (IHC (P))

1:10,000

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:1,000

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PSH06-77

抗原

Synthetic phospho-peptide corresponding to residues surrounding Ser616 of human DRP1.

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偶联物

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Dry ice (international)

产品详细信息

Positive Control: HeLa cell lysate, HeLa treated with 100 ng/mL Calyculin A for 30 minutes cell lysate, NIH/3T3 cell lysate, NIH/3T3 treated with 100 ng/mL nocodazole for 18 hours cell lysate, HeLa, C2C12 cell lysate, PC-12 cell lysate, mouse brain tissue, Jurkat cell lysate, HEK-293 cell lysate, Human brain tissue lysate, Mouse brain tissue lysate, Mouse heart tissue lysate, Rat brain tissue lysate, Rat heart tissue lysate, rat brain tissue, PC-12.

Tissue Specificity: Low tissue specificity.

Subcellular Location: Cytoplasm, cytosol, Golgi apparatus, Endomembrane system, Mitochondrion outer membrane, Peroxisome, Membrane, clathrin-coated pit, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.

Sequence Similarities: 100% Mouse/Rat.

Predicted band size: 82 kDa.

靶标信息

A human dynamin-related protein, DRP1 contributes to mitochondrial division in mammalian cells. It plays this important role in mitochondrial fission at steady state and during apoptosis. DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses, thus providing a genetic tool to assess the role of mitochondria at synapses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: C-terminal region; Dnm1p/Vps1p-like protein; DVLP; Dymple; dynamin 1-like; Dynamin family member proline-rich carboxyl-terminal domain less; Dynamin-1-like protein; Dynamin-like protein; dynamin-like protein 1; Dynamin-like protein 4; Dynamin-like protein IV; Dynamin-related protein 1; DYNIV 11; DYNIV-11; EC 3.6.5.5; FLJ41912; HdynIV; N-terminal region; VPS1

基因别名: 6330417M19Rik; AI450666; DLP1; DNM1L; Dnml1; Dnmlp1; DRP1; DVLP; DYMPLE; EMPF; HDYNIV; python

UniProt ID: (Human) O00429, (Rat) O35303, (Mouse) Q8K1M6

Entrez Gene ID: (Human) 10059, (Rat) 114114, (Mouse) 74006

功能

GTPase activity GTPase activator activity protein binding GTP binding lipid binding Rab GTPase binding GTP-dependent protein binding ubiquitin protein ligase binding protein homodimerization activity identical protein binding nucleotide binding hydrolase activity membrane traffic protein

参与通路

mitochondrial fission release of cytochrome c from mitochondria dynamin polymerization involved in mitochondrial fission endocytosis cellular component disassembly involved in execution phase of apoptosis regulation of mitochondrion organization peroxisome fission regulation of protein oligomerization positive regulation of apoptotic process positive regulation of GTPase activity mitochondrial fragmentation involved in apoptotic process positive regulation of protein secretion protein oligomerization protein homotetramerization heart contraction membrane fusion necroptotic process mitochondrion morphogenesis protein localization to mitochondrion positive regulation of mitochondrial fission mitochondrial membrane fission positive regulation of release of cytochrome c from mitochondria regulation of peroxisome organization regulation of mitophagy regulation of ATP metabolic process positive regulation of intrinsic apoptotic signaling pathway metabolic process programmed cell death