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Phospho-EPRS (Tyr377) Antibody (PA5-105476) in ICC/IF
Immunocytochemistry analysis of Phospho-EPRS (Tyr377) in Hela cells (4h of LPS treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-EPRS (Tyr377) polyclonal antibody (Product # PA5-105476) 1:200 and mouse anti-beta tubulin antibody (1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat ... View More
产品信息
PA5-105476
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
A synthesized peptide derived from human EPRS(Accession P07814), corresponding to amino acid residues around phosphorylated Tyr377.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
sequential chromatography
保存液
PBS, pH 7.4, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C
运输条件
Wet ice
RRID
AB_2816904
产品详细信息
Antibody detects endogenous levels of EPRS only when phosphorylated at Tyr377.
靶标信息
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. EPRS is a multifunctional aminoacyl-tRNA synthetase that catalyzes the aminoacylation of glutamic acid and proline tRNA species.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Bifunctional aminoacyl-tRNA synthetase; Bifunctional glutamate/proline--tRNA ligase; Cell proliferation-inducing gene 32 protein; glutamate tRNA ligase; Glutamatyl-prolyl-tRNA synthetase; glutamine-proline-tRNA synthetase; glutaminyl-tRNA synthetase; proliferation-inducing gene 32 protein; proliferation-inducing protein 32; proline tRNA ligase; proline-tRNA ligase; prolyl-tRNA synthetase
基因别名: 2410081F06Rik; 3010002K18Rik; C79379; EARS; EPRS; EPRS1; GLNS; GLUPRORS; PARS; PIG32; QARS; QPRS
UniProt ID: (Human) Q6PD57, (Mouse) Q8CGC7
Entrez Gene ID: (Human) 2058, (Mouse) 107508
glutamate-tRNA ligase activity
proline-tRNA ligase activity
protein binding
ATP binding
RNA stem-loop binding
GTPase binding
nucleotide binding
RNA binding
catalytic activity
aminoacyl-tRNA ligase activity
ligase activity
ligase activity, forming aminoacyl-tRNA and related compounds
aminoacyl-tRNA synthetase
